Citric acid

Melanin is the natural color in our skin is synthesized by melanocytes. The distribution pattern in the nearby keratinocytes and the nature of the formed melanin determine the actual color of our skin. Melanin form through the series of oxidative reaction involving the amino acid tyrosine in the presence of enzyme tyrosinase. Skin whitening agent after inhibit the activity of tyrosinase. The drawback of the quick effect was, the high cytotoxicity of hydroquinone and its huge irritation potential. So that’s why herbal medicines are currently in demand and their popularity is increasing day by day. The objective of this research is to formulate skin whitening formulation, which have ability to give whitening effect without any side effects using herbal products. Now a day’s hyperpigmentation is a major problem. As per previous literature survey it is reported that lycopene, Citric acid & Tocopherol shows significant result in skin whitening activity. It is reported that plants Tomato, Lemon & Cucumber (fruits) contain high amount of Lycopene, Citric acid & Tocopherol. So from above conclusion to hypothesize that this plants may have significant role in skin whitening. 

A simple, accurate, novel, safe and precise method has been developed for estimation of poorly water soluble drug Ziprasidone Hydrochloride using 2 M Citric acid as hydrotropic agent. After solubilizing the Ziprasidone Hydrochloride in selected hydrotropic agent, it was scanned in spectrum mode and the working wavelength for the estimation, considering the reproducibility and variability was found to be 314 nm. The developed method was found to be linear in the range of 20-100 μg/ml with correlation coefficient (r2) of 0.9997. The mean percent label claims of tablets of ZIP in formulation-I and formulation-II estimated by the proposed method were found to be 97.92±0.59 and 98.46±0.39 respectively. These values are close to 100, indicating the accuracy of the proposed analytical method. Presence of hydrotropic agent do not shows any significant interference in the spectrophotometric assay thus further confirming the applicability and reproducibility of the developed method. The developed methods were validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values.