Chromatography

Current study has developed two precise and direct RP-HPLC approaches for quantitative investigation of glimepiride (GLM) in both mass and pharmaceutical formulations. Glimepiride was analyzed using the RP-HPLC method with C-18 stationary phase and mobile phase of methanol and phosphate buffer (PBS) at pH 4.0 in equivalent volume ratio. The location was established at 239 nm wavelength, and the adaptable stage was extracted at a rate of 0.5 mL/mi. The retention time was observed at 2.470 minutes. Present approach was authenticated in terms of linearity, accurateness, precision, system applicability, limit of detection (LOD), limit of quantification (LOQ), robustness, and ruggedness. It has been demonstrated that the suggested approach is appropriate for monotonous examination of glimepiride in dose and bulk forms, yielding precise results. This method was employed to determine a compound's concentration in commercial pharmaceutical dosage forms. In comparison to alternative chromatographic techniques, this method is more direct, precise, and reproducible, rendering it a superior choice for routine quality control.

The evaluation of cosmetic products has evolved significantly in recent years, driven by the growing demand for safe, effective, and consumer-friendly formulations. Traditional sensory analysis, which relies on human perception of attributes such as texture, fragrance, spreadability, and overall acceptability, continues to play a crucial role in understanding consumer preferences. However, the limitations of subjectivity and variability have encouraged the integration of advanced instrumental techniques. Modern instrumental methods—including rheology, texture analysis, colorimetry, spectroscopy, chromatography, and imaging technologies—provide objective, quantifiable, and reproducible data that complement sensory evaluations. Together, these approaches enable a more comprehensive assessment of cosmetic performance, stability, and consumer satisfaction. This synergistic use of sensory and instrumental methods not only enhances product development and quality control but also supports regulatory compliance and innovation in the cosmetics industry. The paper highlights recent advances, methodological improvements, and the importance of combining instrumental precision with sensory insights for holistic cosmetic evaluation.

The intention of this paper was to review and discuss the some of the quantitative analytical methods used in forensic science for the analysis of trace evidences collected from the various scene incidents. The forensic scientists were depend upon the instrumental analysis of trace amounts of materials like drugs, toxicological specimens, fibers, glass, GSR, soil, etc. Differences in manufacture of chemical composition are observed and that allows considerable discrimination even with very small traces of fragments. Most on GSR, GSR analysis sometimes becomes very important to right direction of inquiry, from where the GSR is obtained and from which surfaces the GSR has produced, played an important aspect of investigations. Reviews on these techniques, which are used more extensively in forensic sciences, were reported through this paper. Based on the analytical problem facing forensic scientists, our study will summarize and use these techniques to tackle them such as chromatography, Raman spectroscopy, Atomic Absorption Spectroscopy, Inductively Coupled Plasma (ICP) techniques.

In the pharmaceutical industry an impurity is considered, defined the any other organic material besides the drug substance or pharmaceutical ingredients.  The impurity may be formed during the formulation or upon aging of two APIs in medicines. The highly sophisticated instrumentation, such as mass spectra meters attached to the gas chromatography or HPLC in various matrices. GC is the most useful technique for identification of residual solvent. The advent of hyphenated technique has revolution any impurity profiling, by not only separation but structural identification of impurities as well among all techniques. The most exploited techniques for impurities profiling of drug are LC-MS-MS, LC-NMR, LC-NMR-MS, GC-MS AND LC-MS.