Biochemical changes

Biochemical changes during in vitro root formation in Tribulus terrestris were investigated in the callus derived from nodal explants on Murashige and Skoog’s (MS) medium supplemented with 1.0 mg/l 2,4-D+ 0.5 mg/l Kinetin. The callus maintained on MS medium fortified with 2.0 mg/l 2, 4-dichlorophenoxy acetic acid (2,4-D) + 0.5 mg/l Kinetin. The above callus subcultured on Murashige and Skoog’s (MS) medium + 2.0 mg/l α-naphthalene acetic acid (NAA). Metabolites like starch and total; soluble sugars decreased while reducing sugar, total phenol and total protein increased during root differentiation in callus. Enzyme activity viz. α-amylase, acid invertase, peroxidase and acid phosphatase increased during root differentiation.