Area under curve

The current work is carried out to for estimation of Chlorcyclizine Hydrochloridein bulk by utilizing area under curve (AUC) method using UV-Visible spectrophotometry. For this purpose the wavelength range 220-240nm was selected. Distilled water was used as a solvent throughout the work. Linearity was observed in concentration range 5-25µg/ml (R2=0.996) for the method. The present method was found to be simple & linear which can be used for routine quality control analysis for spectrophotometric estimation of Chlorcyclizine Hydrochloride in bulk.

The aim of present work was to develop an accurate, precise, reproducible and economical UV spectrophotometric method for estimation of 1H, 1’-H-2, 2’-Bibenzimidazole Impurity In Telmisartan Bulk and Formulation. This method was based on area under curve of UV spectrum between 235 to 254 nm and validated as per ICH guideline Q2 (R1). The method has followed linearity in the range of 5-30μg/ml. The value of correlation coefficient was 0.998. Satisfactory values of Percent relative standard deviation for the intra-day and inter-day precision indicated that method is precise. Results of the recovery studies (97.63% to 98.66 %) showed accuracy of the method. LOD and LOQ were calculated as 0.3221μg/ml and 0.9761 μg/ml, respectively. The developed method can be used for routine estimation of 1H, 1’-H-2, 2’-Bibenzimidazole Impurity In Telmisartan Bulk and Formulation.

The present work describes three simple, precise and economical UVmethods have been developed for the estimation of Maraviroc in bulk and pharmaceutical dosage form. Method A shown the absorbance maxima at 217 nm, method B area under curve method which involves the calculation of integrated value of absorbance with respect to the wavelength between two selected wavelength 212 -222 nm respectively. and in the first order derivative spectra showed sharp peak at 248 nm for method C. The developed methods were founded to be linear in the concentration range of 10-100 µg/ml. The developed methods were validated by following the analytical performance parameters suggested by ICH. All the validation parameters were within the acceptable range. As economical solvent is used, these methods were used for routine analysis of Maraviroc in bulk and pharmaceutical formulation. Key words: Absorption Maxima, Area under curve, Derivative Spectroscopy, Maraviroc, Maraviroc Tablet Dosageform.

The aim of present work was to develop an accurate, precise, reproducible and economical UV spectrophotometric method for estimation of Loratadine. This method was based on area under curve of UV spectrum between 241 to 251 nm and validated as per ICH guideline Q2 (R1). The method has followed linearity in the range of 5-30µg/ml. The value of correlation coefficient was 0.999. Satisfactory values of Percent relative standard deviation for the intra-day and inter-day precision indicated that method is precise. Results of the recovery studies (99.66 % to 99.95 %) showed accuracy of the method. LOD and LOQ were calculated as 0.581 µg/ml and 1.935 µg/ml, respectively. The developed method can be used for routine estimation of Loratadine in bulk and tablet dosage forms.