Antifungal activity.

Synthesis of phenyl (5-substituted phenyl-1,3,4-thiadiazol-2-yl) methanediamine (TDZ-A to TDZ-C), reaction between aryl aldehydes and Thiosemicarbazide yielded thiosemicarbazone. Thiosemicarbazone in the presence of citric acid and sodium acetate gives 2-amino-5-aryl -1,3,4-thiadiazole, which is treated with aniline in the presence of formaldehyde to obtained targeted compounds phenyl(5-substituted phenyl-1,3,4-thiadiazol-2-yl)methanediamine. The synthesized Thiadiazoles have been characterized on the basis of analytical spectral data. The resulted compounds were screened for their antibacterial, antifungal and antioxidant activities.

The present study was aimed to investigate the antibacterial and antifungal activity of aqueous, methanol, ethanol, petroleum ether and acetone extract of canthium coromandelicum using disc diffusion method. The antibacterial activity was detected against Gram-positive and Gram-negative bacteria, Bacillus subtilis, Bacillus ceresu, Staphylococus aureus, Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa. Different solvent extracts of canthium coromandelicum was taken in 100,150,200 and 250 μl/ml. The zones of inhibitions obtained were recorded and analyzed against standard controls, Streptomycin and Amphotericin. The methanolic extract of canthium coromandelicum was showed highest antibacterial activity than ethanolic and aqueous extracts. The higher concentration of methanolic leaves extract of canthium coromandelicum plant, maximum antibacterial activity was observed against to Gram-negative bacteria than Gram-positive bacteria and antifungal activity against to Candida lbicans,  Aspergillus niger. The results indicate that canthium coromandelicum leaves also has potent antibacterial  and antifungal activity.  

Several pharmacological activities like antitubercular, analgesic, anti-cancer, anti-inflammatory, antiasthmatic, antioxidant and antibacterial activities have been attributed to pyrazoles. The above observations prompted us to synthesize some novel pyrazole derivatives as possible antimicrobial agents. A series of novel 1,3,5-trisubstituted pyrazole derivatives (P1-P15) have been synthesized by the reaction of substituted chalcones (C1-C15) with succinichydrazide. The starting material, chalcones were prepared by claisen Schmidt condensation of acetophenone with aldehydes in the presence of sodium hydroxide in ethanol. Succinichydrazide was synthesized by condensing succinic acid with hydrazine hydrate. The cycloaddition of chalcones with succinichydrazide gives 1,3,5-trisubstituted pyrazole derivatives. The structures of synthesized derivatives were confirmed by IR, 1HNMR and Mass spectrum. The synthesized compounds were screened for their antibacterial and antifungal activity. The antibacterial activity data of the synthesized derivatives revealed that the compound P4, P13 and P7, P14 were effective against gram positive and gram negative organisms respectively. The antifungal activity data revealed that the compound P7 and P8 showed good activity against tested fungi.