Vani Pusapati

An accurate, rapid, specific and stability indicating ultra performance liquid chromatographic method was developed and validated for the simultaneous estimation of Verapamil and Trandolapril in a combined pharmaceutical dosage form. The chromatographic separation was attained on Phenonemex Luna C18 (4.0 x 100 mm, 2.6mm) column by isocratic mode with the mobile phase components as 0.03M monobasic potassium phosphate buffer (pH6.5) and acetonitrile (70: 30v/v) at a flow rate of 1.0 mL/min and quantified at 210 nm. The average retention times for Trandolapril and Verapamil were 0.60 and 1.14 min, respectively. The UPLC method proposesoutstanding separation of two drugs with a good resolution of greater than 2.0 and tailing factor less than 2.0 with a run time of 3 minutes. The method shows linearity over the concentration range of 9-45 µg/mL for Verapamil and 0.1-0.5 µg/mL for Trandolapril with a correlation ≥ 0.999. The method is accurate with recoveries in the range of 98.0 -101.0% and precise with %RSD value lesser than2.0% for both the drugs. This method is very fast, cost saving, accurate and specific for the assay of commercially available tablets.

An accurate, rapid, selective and specific reverse phase high performance liquid chromatographic method was developed for the simultaneous estimation of Rosuvastatin and Clopidogrel in a combined formulation. Chromatographic separation was achieved on Inertsil ODS 3 C-18 (250mm x 4.6mm,5µm) column by isocratic elution mode with three mobile phase components, 0.05M potassium phosphate buffer (pH4.2): methanol: acetonitrile (60: 30:10 v/v/v) at a flow rate of 1.0 mL/min and quantified at 238 nm. The average retention times for Rosuvastatin and Clopidogrel were 4.57 and 2.96 min, respectively. The method offers excellent separation of two drugs with resolution > 2.0 and tailing < 1.0 and with no interferences from the excipients. The method is linear over the concentration range of 3.1-18.6 µg/mL for Rosuvastatin and 22.68-136.08 µg/mL for Clopidogrel with a correlation not less than 0.999. The method is accurate with recoveries of both the drugs in between of 98.0 -101.0% and precise with %RSD value less than 2.0% for the Assay of both the drugs. This method is simple, rapid, accurate and specific for the assay of commercial capsules.