Sridevi P

A simple, precise, and accurate UV spectrophotometric method was developed and validated for the simultaneous estimation of Lornoxicam and Thiocolchicoside in bulk and pharmaceutical formulations. The method employs methanol as solvent, and absorbance was measured at the respective wavelengths where both drugs showed maximum absorbance with minimal interference. Calibration curves were linear within the concentration ranges of 4–20 µg/mL for Lornoxicam and 5–25 µg/mL for Thiocolchicoside, with correlation coefficients (R²) of 0.9992 and 0.9995, respectively. Validation was carried out according to ICH Q2(R1) guidelines, including parameters such as linearity, accuracy, precision, LOD, LOQ, robustness, and ruggedness. Recovery studies at 50%, 100%, and 150% levels showed recoveries between 98.7–100.1%, indicating high accuracy. The proposed method is suitable for routine quality control of combined dosage forms of Lornoxicam and Thiocolchicoside.

This study reports the method development and validation for anti-diabetic drugs by UPLC.  A new, simple, rapid, selective, precise and accurate ultra performance liquid chromatography assay has been developed for simultaneous estimation of Remogliflozin, Vildagliptin and Metformin. The separation was achieved by using ODS 3V column with dimensions 5 µm, 4.6 mm x 250 mm. The pH of mobile phase was adjusted to 4.5 with acetonitrile. The flow rate was 0.4 mL/min, and the separated drugs were detected using UPLC detector at the wavelength of 260 nm. The method was validated as per ICH guidelines. The proposed method was found to be accurate, reproducible, and consistent. It was successfully applied for the analysis of these drugs in marketed formulations and could be effectively used for the routine analysis of formulations.