Singh M

Biochemical changes during in vitro root formation in Tribulus terrestris were investigated in the callus derived from nodal explants on Murashige and Skoog’s (MS) medium supplemented with 1.0 mg/l 2,4-D+ 0.5 mg/l Kinetin. The callus maintained on MS medium fortified with 2.0 mg/l 2, 4-dichlorophenoxy acetic acid (2,4-D) + 0.5 mg/l Kinetin. The above callus subcultured on Murashige and Skoog’s (MS) medium + 2.0 mg/l α-naphthalene acetic acid (NAA). Metabolites like starch and total; soluble sugars decreased while reducing sugar, total phenol and total protein increased during root differentiation in callus. Enzyme activity viz. α-amylase, acid invertase, peroxidase and acid phosphatase increased during root differentiation.

  Technology transfer plays a vital role in the process of drug discovery to the product development and the full scale commercialization. The article attempts to discuss about the technology transfer process, steps involved in technology transfer, reasons for using technology transfer, importance of technology transfer and the issues involved in the technology transfer in the pharmaceutical industry.