Rajat Arora

Current study has developed two precise and direct RP-HPLC approaches for quantitative investigation of glimepiride (GLM) in both mass and pharmaceutical formulations. Glimepiride was analyzed using the RP-HPLC method with C-18 stationary phase and mobile phase of methanol and phosphate buffer (PBS) at pH 4.0 in equivalent volume ratio. The location was established at 239 nm wavelength, and the adaptable stage was extracted at a rate of 0.5 mL/mi. The retention time was observed at 2.470 minutes. Present approach was authenticated in terms of linearity, accurateness, precision, system applicability, limit of detection (LOD), limit of quantification (LOQ), robustness, and ruggedness. It has been demonstrated that the suggested approach is appropriate for monotonous examination of glimepiride in dose and bulk forms, yielding precise results. This method was employed to determine a compound's concentration in commercial pharmaceutical dosage forms. In comparison to alternative chromatographic techniques, this method is more direct, precise, and reproducible, rendering it a superior choice for routine quality control.