Prakash.V. Diwan

A simple, sensitive, accurate and precise simultaneous UV spectrophotometric method has been developed for the estimation of Ibuprofen, Paracetamol and Caffeine in tablet dosage form. The absorption maxima of the drugs were found to be 223, 248 and 272 nm for Ibuprofen, Paracetamol and Caffeine respectively, in methanol, using a Shimadzu UV–Visible spectrophotometer (model UV-1800). Ibuprofen, Paracetamol and Caffeine obeyed Beer’s law in the concentration range of 10-70 µg ml-1, 10-60 µg ml-1 and 10-70 µg ml-1 respectively. The correlation coefficient was found to be 0.999, 0.999, and 0.999 for Ibuprofen, Paracetamol and Caffeine respectively. The method was validated for various parameters according to ICH guidelines. The low relative standard deviation values indicate good precision and high recovery values indicate accuracy of the proposed method. Assay results were in good agreement with label claim.

A simple, rapid and specific UV spectrophotometric method with good sensitivity was developed and validated for the simultaneous determination of tamsulosin and finasteride in bulk and pharmaceutical formulations. In methanol, the lambda max of finasteride and tamsulosin was fixed as 235 and 225nm respectively, using a Shimadzu UV–Visible spectrophotometer (model UV-1800) with quartz cells. In this proposed method both these drugs obeyed linearity individually and in mixture within the concentration range of 1- 10 μg ml-1 for tamsulosin and 12.5 - 100 μg ml-1 for finasteride, with a correlation coefficient value of  0.9992 and 0.9994 for tamsulosin and finasteride respectively. The low relative standard deviation values indicate good precision and high recovery values indicate accuracy of the proposed method. The proposed method had been applied to the determination of drugs in commercial formulations. Assay results were in good agreement with label claim. The method was validated according to the ICH guidelines.