Meera Sum

This study was taken up to assess the aqueous and ethanol extracts of whole plant of Potentilla anserina Linn for anti-inflammatory activity in rats by Formalin induced paw oedema method. Animals were divided into 6 groups of 6 rats. Groups 1 and 2 served as Formalin induced control and standard (Diclofenac 5 mg/Kg, i.p.) respectively. Groups 3 and 4 were treated, orally with aqueous extract of Potentilla anserina Linn of 100 and 400 mg/kg b.w, respectively. Groups 5 and 6 were administered, orally with ethanol extract of Potentilla anserina Linn of 100 and 400 mg/kg b.w, respectively. The paw volume was measured at 0, 1, 2, 3 and 4 hr after Formalin injection. The actual edema volume was calculated. The data was expressed as mean ± S.E.M. The statistical analysis was done by means of ANOVA followed by Dunnett’s post hock test. The aqueous and ethanol extracts of whole plant of Potentilla anserina Linn produced significant reduction of edema in the rats. Ethanol extract of whole plant of Potentilla anserina Linn revealed significant anti-inflammatory activity.

This study was designed to evaluate, aqueous and ethanol leaf extracts of Barleria longiflora L.f. for anti-inflammatory activity using Formalin induced paw oedema in rats. Animals were divided into 6 groups of 6 rats. Groups 1 and 2 served as Formalin induced control and standard (Diclofenac 5 mg/Kg, i.p.) respectively. Groups 3 and 4 were treated, orally with aqueous extract of Barleria longiflora L.f. of 100 and 400 mg/kg b.w, respectively. Groups 5 and 6 were administered, orally with ethanol extract of Barleria longiflora L.f. of 100 and 400 mg/kg b.w, respectively. The paw volume was measured at 0, 1, 2, 3 and 4 hr after Formalin injection. The actual edema volume was calculated. The data was expressed as mean ± S.E.M. The statistical analysis was done by means of ANOVA followed by Dunnett’s post hock test. The aqueous and ethanol leaf extracts of Barleria longiflora L.f. exhibited significant reduction of edema in the rats. Ethanol leaf extract of Barleria longiflora L.f. revealed surpassing anti-inflammatory activity.

This survey was designed to investigate the evaluation of synergic anticancer activity of Punica grantum L and Ziziphus mauritiana extracts by using invitro cytotoxicity assay, invitro and invivo antioxidant estimation. The invitro antioxidant activity of ethanol, aqueous and chloroform extracts of  Punica grantum L and Ziziphus mauritiana and combination of them was estimated by reducing power, diphenyl picryl hydrazyl scavenging and hydrogen peroxide scavenging assays using standard procedure, the invivo antioxidant activity was  also performed  by estimation of  Catalase and Glutathione in liver homogenate. The extracts were screened for their invitro cytotoxicity by trypan blue exclusion assay and Allium cepa root tips. The results displayed the 100µg/ml of both ethanol and aqueous extracts i.e. Punica grantum L and it’s combination with Ziziphus mauritiana having the maximum invitro antioxidant activity. Ethanol and aqueous extract of combination of Punica grantum L and Ziziphus and ethanol extract of Punica grantum L has produced the significant increase in the glutathione and catalase levels. The cytotoxic effect of ethanol extracts of Punica grantum L and Ziziphus mauritiana significantly increased in Ehrlich ascites tumour cells with an increase in concentration by trypan blue cytotoxic assay and also significant root growth inhibition in Allium cepa after 48h. The percentage decrease in mitotic index was found to be 27.28 % in 800 μg/ml of ethanol extracts of Punica grantum L and Ziziphus mauritiana. Accordingly, the synergic anticancer activity of illustrated extracts was evinced. Key words: Antioxidant, cytotoxic assay, Punica grantum L, Zizipus mauritiana