M. M. Eswarudu

Hypertension (HT) is a very common disorder, particularly for past middle age. It is not a disease in itself, but is an important risk factor for cardiovascular mortality and morbidity. For improved treatment of hypertension, Telmisartan and Benidipine HCl is the newer combination in market, this combination was developed to improve medication for Stage II hypertension. The aim of this review is to focus on comprehensive update of different analytical methods used for estimation of anti-hypertensive drugs like Telmisartan and Benidipine HCl for the treatment of hypertension. This review delivers a detail description on different analytical methods like UV and RP-HPLC for Telmisartan and Benidipine HCl individually and combination with other drugs. For this review, data searches were conducted by scientific papers in the literature as well as in official compendium. All reported methods are found to be simple, accurate, economic, precise and reproducible in nature.

A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed and validated for the estimation of Venlafaxine hydrochloride in pure and Pharmaceutical dosage form. Kromasil C18 column having 150 mm x 4.6 mm internal diameter, 5 µm particle sizes in isocratic mode with mobile phase containing mixture of methanol and water in the ratio of 65:35 v/v was used. The flow rate was 1.0 ml/min and effluents were monitored at 225 nm. The retention time for Venlafaxine was 2.424 min. The method was validated for linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness. Limit of detection and limit of quantification were found 2.97µg/ml and 9.92 µg/ml respectively and recovery of Venlafaxine from tablet formulation was found 100.4 %. The proposed method was successfully applied for the quantitative determination of Venlafaxine in tablet dosage form.