Komal Patel

A simple, precise and accurate RP-HPLC method was developed for the separation and quantification of  Ilaprazole and Levosulpiride in pharmaceutical dosage form. The quantification was carried out using Hypersil BDS C8 column ( 150x 4.6mm,5µm) and mobile phase comprised of methanol and phosphate buffer(pH 7.5 adjusted with 0.01 M NaOH) in proportion of ratio 70:30 degassed under ultra- sonication. The flow rate was adjusted to 1 ml/min and the effluent was monitored at 242nm using PDA detector. The method was validated in terms of linearity, precision, accuracy and specificity, limit of detection and limit of quantization. Linearity of Ilaprazole and Levosulpiride were in range of 10-60 µg/ml and 75-450 µg/ml respectively. The retention time of Ilaprazole and Levosulpiride were 6.52 and 2.02 respectively. The percentage recoveries of both drugs were found to be 100.03%  and 100.18% for Ilaprazole and Levosulpiride respectively from the capsule formulation. The method was found to be precise, accurate and specific during the study. The proposed method enables rapid quantification and simultaneous analysis of both drugs from commercial formulations without any excipients interference. The method can be used for routine analysis of marketed product of Ilaprazole and Levosulpiride in combined capsule formulation.

A simple, accurate, precise, reproducible and economical UV spectroscopic methods for simultaneous estimation of Flupentixol and Melitracen in tablet dosage form have been developed. simultaneous equation method employs formation and solving of mathematical simultaneous equation using 254 nm and 230 nm as the λmax of Melitracen and Flupentixol respectively in 0.1N HCl. These methods were validated as per ICH norms. Calibration curves were linear over the concentration ranges of 1-10 μg/ml for Flupentixol and 10-100 μg/ml for Melitracen with mean recovery of 100.71 ± 1.53 & 100.36 ± 0.66 for Flupentixol & Melitracen respectively by Simultaneous Equation method. The validation study is statistically significant as all the statistical parameters are within the acceptance range (% RSD < 2.0 and S.D. < 2.0) for both accuracy and precision. The methods are successfully applied to pharmaceutical formulation, with no interference from excipients as indicated by the recovery study. The proposed methods are simple, rapid, economic and accurate for routine simultaneous estimation of Flupentixol and Melitracen.

A simple, rapid, accurate, precise and reproducible reverse phase high performance liquid chromatographic method has been developed for the estimation of Risperidone and Trihexyphenidyl hydrochloride was determined using reversed-phase liquid chromatography method using ODS Hypersil C18 column (250 mm × 4.6 mm id, 5μm as a stationary Phase and Methanol : Acetonitrile : Acetate Buffer (pH 4.0) (70 : 20 : 10, v/v/v) as a mobile phase pumped at a flow rate of 1.0 ml/min. Quantification was achieved with ultraviolet detection at 214 nm over concentration ranges of 2-20 μg/ml for Risperidone and 1-10 μg/ml for Trihexyphenidyl hydrochloride with mean accuracy 101.02 ± 0.19 and 101.3 ± 0.38 %, for Risperidone & Trihexyphenidyl hydrochloride respectively. The method was successively applied to tablet dosage forms as no chromatographic interferences from the tablet excipients were observed. The method retained its accuracy and precision when the standard addition technique was applied.