Dipti

A rapid, simple, selective, and specific reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for estimation of Naproxen and Sumatriptan from tablet using spiked human plasma. The chromatographic separation was performed on Phenomenex Luna C18 column (5μm, 25cmx4.6mm id)  with a mobile phase comprised of Acetonitrile: Methanol: phosphate buffer pH 6 (50:10:40 v/v), at a flow rate of 1.0ml/min. The calibration curve was linear in the range of 1-3 µg/ml. The developed method was found to accurate and sensitive. Results of recovery studies prove the extraction efficiency. Stability data indicated that Sumatriptan and Naproxen was stable in plasma after three freeze thaw cycles and upon storage at −20°C for 30 days.

Vasopressin, nonapeptide, used as an antidiuretic hormone. Very few method has been reported for analysis of Vasopressin from pharmaceutical dosage form. A simple and rapid high performance liquid chromatography (HPLC) method was developed for the quantitative analysis of arginine vasopressin released from polymeric nanoparticles. Chromatographic analysis was performed on an RP C18 column with a mobile phase consisting of acetonitrile and phosphate buffer (13:87 v/v) at a flow rate of 1.6 ml/min at a wavelength of 220 nm,  with a retention time 4.1 min. The method was shown to be specific and linear in the range of 1-50 IU/ml (r2 = 0.9997). Developed method was validated for various evaluation parameters as per ICH guidelines. The method showed no peak interference in presence of formulation excipients. The limit of detection and quantitation were 0.32 and 1.06 IU/ml, respectively. The method was applied to the quantitative analysis of drug to study in vitro drug release from polymeric nanoparticles.

Four diverse genotypes of Indian mustard (Brassica juncea L. Czern & Coss.)  and their crosses with Ogura restorer as the background were evaluated for eleven quantitative traits during winter (Rabi) 2011–12. The mean, range, phenotypic, genotypic and environmental variance, genotypic and phenotypic coefficient of variation, heritability in broad sense and genetic advance were calculated to differentiate the contribution made by each parameter in the final seed yield. The maximum value of phenotypic coefficient of variation was observed for no. of pods on main branch (36.23) followed by oil content (31.65), siliqua density on main shoot (30.72) and the highest genotypic coefficient of variation was observed for number of pods on main branch (33.49) followed by seed yield per plant (29.18) followed by oil content (28.89). Path coefficient analysis revealed that no. of pods on main branch (5.94) had the highest direct contribution towards seed yield per plant followed by 1000-seed weight (4.14) and siliqua density on main shoot (1.82) which suggested that selection for these traits would be quite effective in improving seed yield in Indian mustard. During the course of investigations, it was observed that the mean performance of F1’s (OR x RH 406 and OR x RH 832) was better than parental genotypes for no. of primary and secondary branches, no. of seeds per siliqua and seed yield per plant.

A simple, sensitive, rapid, accurate and precise spectrophotometric method has been developed for estimation of Glimepiride in bulk and tablet dosage forms. The zero order spectra shows maximum absorbance at 249 nm. Calibration graph was found to be linear over the concentration range of 5-30 μg/ml. Results of analysis were validated for precision, range, linearity, interference study and recovery studies, The method can be adopted in its routine analysis.

Eye is a window to the outside world and hence it becomes an important part of our body. Various diseases occurring in the eye cavity are difficult to treat. Some diseases require continuous use of medicine and other requires surgery. Lacrisert, Vitrasert, Mydriasert, Prosert etc. are patented technologies useful in the treatment of eye disorders. In this article brief description of various patented technologies such as Eyegiene kit, Ophtha coils, Versidoser, Microneedle, Implantable MEMS ocular drug delivery system, Ocuseal liquid bandage, Filter paper strips is given.

  The most significant synthesis of isoindoles (phthalimides) is the dehydrative condensation of phthalic anhydride at high temperature with primary amines (adenine). Novel isoindole analogues with adenine can be synthesized through condensation of phthalic anhydride or substituted phthalic anhydride and purine (adenine). In this study only one type of isoindole analogues (phthalimides) were synthesized using mono bromo phthalic anhydride with primary amine (adenine). Different Mannich bases of isoindole analogues were synthesized using various types of aliphatic amines with variable yields (60- 80%). These Mannich bases were identified and confirmed by FT-IR, NMR and elemental analysis. The antimicrobial assay of these synthesized analogues was tested against H. pylori, E. coli, P. auroginosa, S. typhi, B. subtilis, B. threogensis, S. aureus, MRSA, A. niger, and were found to be active at Minimum Inhibitory Concentration [MIC] range of 125-1000 µg/ml. The most active compounds in the series was S-3 which has shown antibacterial activity against H. pylori at MIC of 125 µg/ml. This compound was identified as lead compounds and further molecular modification is in progress.