B .Tirumaleswara Rao

A simple, rapid, sensitive reverse-phase high-performance liquid chromatography method was developed and validated for simultaneous estimation of ofloxacin and ornidazole, at single wavelength of 343nm.chromotographic separation was performed on an enable aligent zorabax(thermo) column(250nmx4.6mm ID particle size 5 um) and a  mobile phase consisting of acetonitrile and buffer (600:4300v/v) at a flow rate of 1.0ml/min. the calibration curve was linear(r2≥0.0999) over the concentration range. 400-1200μg/mL of ofloxacin and 1000-3000μg/mL of ornidazole. the limit of detection 0.00246µg/ml  for ofloxacin  0.00508µg/ml for ornidazole and no interference was found by the excipients in the synthetic mixture. The proposed methods were validated for international conference on harmonization guideline for linearity, accuracy, precision, and robustness for estimation of ofloxacin and ornidazole in bulk and synthetic mixture, and The results were found to be satisfactory