Anita

In recent years, the use of computers in chemistry have been developed greatly. The molecular docking, computer based drug designing, etc. has proved the efficiency of computers in computational chemistry. With the use of sophisticated computational tools and techniques the drugs discovery has been accelerated in the form of target based drug discovery rather than rational methods. In the current scenario the software used for drug designing are very expensive, so in this paper the power of internet and open source community has been used for performing SAR and Pharmacophore based drug design approaches as they are free to use. Using SAR and Pharmacophore study here some derivatives of Podophyllotoxin have been designed by understanding their toxicity, metabolic sites and drug like properties.

Concomitant therapy of anti inflammatory agent and disease modifying drug in pulsatile pattern proves the best treatment for pain relief and in remission of disease. In the current work, combination therapy of aceclofenac and leflunomide was studies for rheumatoid arthritis. Tab in tab formulation was developed with inner leflunomide and outer aceclofenac fraction. Outer fraction provides instant relief from symptomatic pain and inner part provides disease remission. pH sensitive polymers were investigated for coating and coating parameters were optimized. Considering the pH solubility of Eudragit S 100 and Eudragit L 100 in different ratio, final composition was derived. With final composition, desired lag phase observed in the drug release study. Further, X-ray studies and Pharmacokinetic study with final formulation also proves the efficacy of the final formulation w.r.t. desired lag phase and drug plasma concentration in-vivo. Final formulation found stable in 3 months stability study w.r.t. physical and chemical parameters. Optimized novel pulse release combination therapy developed to provide the relief to arthritis patient. 

Aegle marmelos, a plant having tremendous therapeutic potential, the importance seems largely due to its medicinal properties and all the parts viz. roots, leaves, fruits, bark and seeds are used for curing human ailment. In the present investigation, the anticlastogenic potential of hydromethanolic Aegle marmelos (AM) leaves extract has been evaluated using in vivo Micronucleus assay in Swiss albino mice. Cyclophosphamide (CP), a well known mutagen was given intraperitoneal (i.p.) injection at the dose of 50 mg/kg bodyweight (b.w.). AM leaves extract at the doses of 450, 675, 900 mg/kg b.w. provided protection when given 24 hrs. prior to CP administration. In CP treated animals, a significant induction of micronucleus was recorded and in different AM extracts supplemented groups, a dose dependent significant decrease in CP induced clastogenicity was observed which was statistically significant (p

The present work reports on the preparation of ethyl cellulose (EC)-pluronic F127 (PF127)-based tableted floating microspheres by the oil-in-water emulsion solvent evaporation method for the controlled release of acyclovir (ACV). Microspheres of this study were characterized by Fourier transform infrared (FTIR) spectroscopy to investigate the chemical interactions of ACV with the polymer, floating behavior, scanning electron microscopy (SEM) for morphology of the microspheres, differential scanning calorimetry (DSC) for investigating their thermal properties  and X-ray diffraction (XRD) as well as. In vitro release experiments of microspheres were performed in acidic pH 1.2 media to understand the release profiles of ACV. The selected sets of microspheres were compressed into tablets using the compressible excipients and their in vitro release performances were evaluated in pH 1.2 media.

There is highly co-incidence between hypertension and insulin resistance which is the important causative factor to develop diabetes mellitus (DM). Our study was prospective study in effect of beta-blockers, angiotensin-converting enzyme inhibitor (ACEI) and calcium channel blocker (CCB) on blood glucose level in patients newly diagnosed with hypertension and without diabetes in MVJ hospital, Bangalore. Fasting blood glucose and post prandial blood glucose measured for patients prescribed antihypertensive for four months. In a nutshell, we observed that Beta blockers worsen the glycemic condition and it increased blood glucose level, beside that it increased incident of impaired glucose tolerance. In case of calcium channel blocker and ACEIs, blood glucose level slightly increased and decreased respectively after two months.    

Nateglinide, an oral hypoglycemic agent is having disadvantage of low systemic bioavailability, poor absorption in upper intestinal tract, and short biological half life (1.5hr) for increasing the resident time of this drug in stomach spray drying technique used for preparation of mucoadhesive microspheres consist of different polymers like Hydroxypropyl Methycellulose (HPMC), Hydroxypropyl cellulose (HPC), Polyvinylpyrrolidone (PVP), Sodium alginate and ethyl cellulose with Nateglinide. The surface morphology and particle shape were studied by scanning electron microscope (SEM). The microspheres were evaluated for their micro encapsulation efficiency. The micro encapsulation efficiency of microspheres evaluated. Mucoadhesive microspheres prepared were spherical in shape, size in the range of 2.6-5μm.In vitro drug release performed for all the formulation and in vivo study of optimum formulation (F3) and pure Nateglinide in normal healthy rabbits performed. The micro encapsulation efficiency was in the range of 76.75 % - 89.36 % and microspheres of formulations (F1, F2, F3, F4, F6, and F7) have shown good mucoadhesive property. F3 had shown significant hypoglycaemic effect upto period from 5 hrs to 19 hrs, whereas pure Nateglinide showed the reduction of 39.85% after a period of 3hrs and reached normal with in 6.5hrs.

The article describes strategy for masking the intensely bitter taste of Azithromycin Dihydrate(AZT) by using complexation with Kyron T-134. The resinates prepared with drug-Kyron T-134 ratio (1:3) at pH 8, gave maximum drug loading. Suspension containing, resinate showed more than 90% in-vitro drug release within 45min. Prepared formulation showed good stability and retention of palatable taste. Thus, the “patient-friendly dosage form” of bitter drugs, especially for pediatric, geriatric, bedridden, and non cooperative patients, can be successfully formulated using this technology.

A simple, precise and economical UV Spectrophotometric method has been developed for the estimation of Tolterodine tartarate in bulk and pharmaceutical dosage form. The method is based on measurement of absorption at maximum wavelength of 283.0 nm. Linearity for detector response was observed in the concentration range of 10-50 μg/ml. The accuracy of the method was assessed by recovery studies and was found to be 99.80%. The LOD and LOQ were found to be 0.1865 and 0.5621 respectively. The developed method was validated with respect to linearity, accuracy (recovery), precision, specificity and robustness and ruggedness. The results were validated statistically as per ICH Q2 R1 guideline and were found to be satisfactory. The proposed method was successfully applied for the determination of Tolterodine tartarate in commercial pharmaceutical dosage form.

In the current status there is no explicit demarcation methodology on finding of substitutes (Pratinidhi Dravya). Thus here an attempt has been made to find out substitute by DNA fingerprinting on the basis of similarity and dissimilarity. In this study two samples Alysicarpus longifolius W. & A.Prodr.- Fabaceae, Desmodium laxiflorum DC i.e. of same family, same genus with different species were subjected to fingerprints. In this data we find a more similarities characteristics in both the plants i.e. up to 63%. As per the knowledge of research scientist this is a first moral attempt which throwing lights on concept of Pratinidhi Dravya (Substitution) in Ayurvedic science.

  The objective of the present investigation was to develop and evaluate microemulsion based gel for the vaginal delivery of Sertaconazole. The solubility of Sertaconazole in various oils, surfactants and co-surfactants were checked to identify components of the microemulsion. The ternary diagrams were plotted to identify the area of microemulsion existence. Various gelling agents were evaluated for their potential to gel the Sertaconazole microemulsion without affecting its structure. Carbopol 940 was selected for the formulation of microemulsion based gel. The prepared formulations of Sertaconazole Microemulsion based gel was evaluated by checking its pH, spreadability, rheological studies, mucoadhesive strength, in-vitro drug release studies and ex-vivo retention studies. The Sertaconazole Microemulsion based gel showed good in vitro bioadhesion and anti-fungal activity. The Sertaconazole Microemulsion based gel has potential be successfully used for the topical treatment of vaginal candidiasis.