QHPTLC Method for the Estimation of Santalum Album In Padoladi Ghritham- A Polyherbal Ayurvedic Formulation

A Quantitative High Performance Thin Layer Chromatography (QHPTLC) method was developed for the estimation of ‘Candana’ (Santalum album), one of the herbal ingredients present in the Ayurvedic polyherbal formulation ‘Padoladi Ghritham’. For this, both the methanol extracts of ‘Candana’ crude drug and Padoladi Ghritham were run using a mobile phase Chloroform : Isopropyl alcohol : Toluene (8:1:1, v/v/v) on a silica G 60 F254 stationary phase. Methanol extract of Candana  gave a spot with Rf value of 0.76. The methanol extract of Padoladi Ghritham also gave a spot with Rf value of 0.76. The chemical equivalency of these two spots was revealed by the same pattern of the UV spectrum with a λmax of 290 nm. Then the estimation was done graphically by preparing Quarter Standard, Half Standard, Normal Standard, and Double Standard of the formulation. A calibration plot was drawn between the concentration of standards and peak area of the reference spots. The concentration of Candana in the samples was found from the corresponding AUC obtained for the reference peak of the samples. A regression coefficient of 0.989 was obtained. The developed QHPTLC method is a simple and accurate one and can be used as a tool for the quantification of each ingredient in a polyherbal formulation.