Development and Validation of RP-LC Method for Curcumin in Pharmaceutical Formulations

A new, simple, rapid, selective, precise and accurate isocratic reverse phase high performance liquid Chromatography assay method has been developed for estimation of Curcumin in tablet formulations. The separation was achieved by using column Hypersil BDS C18, 150x4.6 mm, 5µ (Make: Thermo), in mobile phase consisted of tetrahydrofuran and citric acid buffer in the ratio of (550:450, v/v). The flow rate was 1.0 mL.min-1 and the separated curcumin was detected using UV detector at the wavelength of 425 nm. The retention time of curcumin, was noted to be 8.05 min respectively, indicative of rather shorter analysis time. The method was validated as per ICH guidelines. The proposed method was found to be accurate, reproducible, and consistent.