Development and Validation of First Order Derivative UV Spectrophotometric Method for Simultaneous Estimation of Cefoperazone Sodium and Tazobactam Sodium In Pharmaceutical Formulation

A simple, accurate, reliable and reproducible first order derivative method was developed for the simultaneous determination of Cefoperazone sodium (CEFO) and Tazobactam sodium (TAZO) in pharmaceutical formulation. The linearity was carried out by using the concentration range 5-50 µg/ml for CEFO (275.2 nm ZCP of TAZO) and 2-50 µg/ml for TAZO (225 nm ZCP of CEFO) respectively. The correlation coefficient of CEFO and TAZO was found to be 0.999 and 0.998 respectively. At zero crossing point (ZCP) of CEFO (225nm) TAZO showed a measurable derivative absorbance, whereas at zero crossing point (ZCP) of TAZO (275.20nm) CEFO showed a appreciable derivative absorbance value. Precision study showed that %RSD was within range of acceptable limits (< 2%). The % recovery for CEFO and TAZO was found to be within range of 98-101% and 98-102%respectively. The percentage assay was found to be 101.05% and 99.41% for CEFO and TAZO. The result of analysis has been validated as per ICH guideline. This method has applied successfully for determination of CEFO and TAZO in its pharmaceutical formulation.