Simultaneous Estimation of Paracetamol and Dicyclomine Hydrochloride by Spectrophotometric Method

A simple accurate, precise and reproducible spectrophotometric method has been developed and validated for the simultaneous estimation of Paracetamol and Dicyclomine hydrochloride in combined tablet dosage form by Q-Absorbance ratio method. Q-Absorbance ratio method involves two wavelengths the iso-absorptive point and  λmax of paracetmol i.e. 231.5 nm and 257 nm respectively in 6 : 4 ratio methanol: 0.1 N NaOH mixture.  The linearity was detected in the range of 2-12 µg/ml (R2 = 0.9997) for Paracetamol and 10-35 µg/ml (R2 = 0.9989) for Dicyclomine Hydrochloride. The accuracy existed between 98-102% and the %RSD was less than 2%. The developed method was validated for linearity, accuracy and precision as per ICH guidelines.