Preparation and In-Vitro Evaluation of Polyherbal Formulation for Wound Healing

Wounds are the result of injuries to the skin that disrupt the other soft tissue. Healing of a wound is a complex and protracted process of tissue repair and remodeling in response to injury. Various plant products have been used in treatment of wounds over the years. Wound healing herbal extracts promote blood clotting, fight infection, and accelerate the healing of wounds. Phytoconstituents derived from plants and identified and screened for antimicrobial activity, and the ratio was selected for the preparation of ointment. The in vitro assays are useful, quick, and relatively inexpensive. The wound healing efficacies of various herbal extracts have been evaluated in CAM assay in in-vitro model. CAM  was used to assess the angiogenic activity of polyherbal formulation. Angiogenesis plays an important role in wound healing and newly formed blood vessels comprise 60% of the repair tissue. Neovascularization helps hypoxic wounds to attain the normoxic conditions.  The CAM assay was done in prepared Polyherbal formulation , control and standard. As shown in the figure below the Prepared polyherbal formulation possess the good growth of blood vessels as compared to control and standard. This observation indicates that the developed veterinary polyherbal formulation has good angiogenic property. Improved angiogenesis, therefore, would be contributing significantly to wound healing activity of polyherbal formulation.