Qualitative and Quantitative estimation of Deacylgymnemic acid by HPLC methods in Aqueous extract followed by Hydro alcoholic and Alcoholic extract.

Aqueous Extract Followed By Hydroalcoholic And Alcoholic Extract Gymnema Sylvestre Roxb.1,2 Family Asclepiadaceae And Stevia Rebaudiana Bertoni3amily Compositae. Literature survey reveals that till date, no combination of Stevia rebaudiana and Gymnema sylvestre are available in any dosage form. In the present work, the main objective is to formulate, evaluate and validate anti-diabetic liquid oral preparation of Gymnema using Stevia as sweetener. Stevia, non-caloric natural sweetener has added advantage than artificial sweetener due to number of beneficial effects Successive solvent extraction of Gymnema sylvestre and Stevia rebaudiana were carried out with the solvents like petroleum ether (60-800C), ethanol, hydroalcohol and water. Phytochemical screening of aqueous extract of formulation was performed and their purity was checked by TLC of aqueous extract of Gymnema sylvestre, formulation and deacylgymnemic acid were done. While preparing liquid oral formulation, aqueous extract of Gymnema sylvestre and Stevia rebaudiana were selected, because of their maximum yield and presence of phytochemicals, which were present in alcoholic and hydroalcoholic extract. Stevia rebaudiana is a natural sweetner. In the presented formulation, stevia was used as a sweetner to make the formulation sweet. Validation of liquid oral preparation was done. Physical, chemical evaluations were carried out. The amount of deacylgymnemic acid present was found to be 22.327 mg/ml by HPLC.