Development and Validation of Second Order Derivative Spectrophotometric Method for Simultaneous Estimation of Paracetamol and Pamabrom in Pharmaceutical Dosage Form

The present manuscript describes simple, sensitive, rapid, accurate, precise and economical second derivative spectrophotometric method for the simultaneous determination of Paracetamol and Pamabrom in dosage form. The derivative spectrophotometric method was based on the determination of both the drugs at their respective zero crossing point (ZCP). The second order derivative spectra were obtained in dist. water and the determinations were made at 268.2 nm (ZCP of Pamabrom) for Paracetamol and 225.0 nm (ZCP of Paracetamol) for Pamabrom. The linearity was obtained in the concentration range of 4-18 μg/ml for Paracetamol and 2-16 μg/ml for Pamabrom The method was found to be simple, sensitive, accurate and precise and was applicable for the simultaneous determination of Paracetamol and Pamabrom in pharmaceutical tablet dosage form.