Hardik Padaliya

A simple, rapid and precise Reverse Phase High Performance Liquid Chromatographic method was developed for simultaneous estimation of Eperisone hydrochloride and Diclofenac sodium in pharmaceutical dosage form by reverse phase Pinnacle DB C-18 column (250 mm, 4.6 mm, and 5 μm). The sample was analyzed using 50mM ammonium acetate buffer containing 0.2% triethylamine (pH-4.0 adjusted with glacial acetic acid): Acetonitrile (40:60, v/v), as a mobile phase at a flow rate of 1.0 ml/min. and detection at 273 nm. The retention time for Eperisone hydrochloride and Diclofenac sodium was found to be 3.07 min and 5.56 min, respectively. The linearity of developed method was achieved in the range of 10-100 μg/ml for Eperisone hydrochloride and 10-100 μg/ml for Diclofenac sodium. The method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation, robustness and ruggedness as per ICH guidelines.

The present manuscript describes simple, sensitive, rapid, accurate, precise and economical second derivative spectrophotometric method for the simultaneous determination of Paracetamol and Pamabrom in dosage form. The derivative spectrophotometric method was based on the determination of both the drugs at their respective zero crossing point (ZCP). The second order derivative spectra were obtained in dist. water and the determinations were made at 268.2 nm (ZCP of Pamabrom) for Paracetamol and 225.0 nm (ZCP of Paracetamol) for Pamabrom. The linearity was obtained in the concentration range of 4-18 μg/ml for Paracetamol and 2-16 μg/ml for Pamabrom The method was found to be simple, sensitive, accurate and precise and was applicable for the simultaneous determination of Paracetamol and Pamabrom in pharmaceutical tablet dosage form.