Development and Validation of Solvent Extraction Spectrophotometric Method for Simultaneous Estimation of Doxofylline and Terbutaline sulphate In their Combined Dosage Form

Simple, specific, accurate, precise and reproducible method have been developed and validated for the simultaneous estimation of both drugs in their combined dosage form. UV spectrophotometric method was a determination using the solvent extraction method at 277 nm and 279 nm over the concentration range 10-50 and 20-60 μg/ml for Doxofylline in chloroform and terbutaline sulphate in water respectively. The % recoveries of the both the drugs were found to be 100.34% – 100.72 % and 98.25– 99.19 % respectively. Method was statistically validated for accuracy, precision, specificity, LOQ, robustness and ruggedness according to ICH guidelines and can be used for analysis of combined dosage form. Key words: Doxofylline, Terbutaline sulphate, Solvent Extraction Spectrophotometric method.