Simultaneous Quantification of Umbelliferone and Quercetin in Polyherbal Formulations of Aegle Marmelos by HPTLC.

In the present investigations, methanolic extracts of four marketed Aegle marmelos formulations (F1, F2, F3, and F4) were prepared and subjected to simultaneous quantitative determination of two biologically active compounds; umbelliferone and quercetin. Analysis of umbelliferone and quercetin was performed on TLC aluminum plates pre-coated with silica gel 60F-254 as stationary phase. Linear ascending development was carried out in twin trough glass chamber saturated with mobile phase consisting of toluene: ethyl acetate: formic acid (6:4:1, v/v/v), and densitometric determination of these compounds was carried out at 300nm in reflectance/absorbance mode. The system was found to give compact spots for umbelliferone and quercetin with Rf value of 0.66 and 0.68, respectively. The present method was validated for precision, recovery, repeatability, and accuracy in accordance with International Conference on Harmonisation (ICH Q2) guidelines. Statistical analysis of the data showed that the method is reproducible and selective for estimation of umbelliferone and quercetin. This method may be used for routine quality control and standardization of the herbal drugs and there formulations. Key words: Aegle marmelos, Quercetin, Umbelliferone, HPTLC