Interaction of Serum Proteins In Evaluating the Efficacy of Sevelamer Hydrochloride and Sevelamer Carbonate Together with Dietary Sources In Hyperphosphatemia Condition

Kidney is one of the vital organs in the human body. The function of the kidney involves purification of the blood, reabsorption of water, excretion of waste, secretion of hormones like adrenaline. When the kidney becomes non-functional, it leads to a condition called chronic kidney disease (CKD), where the kidney fails to purify the blood. When this happens, the patients are expected to switch over to artificial methods of purifying the blood such as dialysis. Patients suffering from CKD have high levels of phosphate (hyperphosphatemia) and leads to calcification of blood vessels. In order to reduce phosphate levels the patients are recommended drugs which act as phosphate binders like sevelamer hydrochloride and sevelamer carbonate. In the present investigation the banding patterns of serum proteins and the serum along with plant samples and the drug were analysed through Native –PAGE. The serum protein interaction studies showed a positive result for sevelamer hydrochloride where as the sevelamer carbonate displayed less interaction. The swelling property of the drug was studied using phase contrast microscopy. It was observed that the drug swells up when treated with water and also the content of the drug slowly dissolves with time. Keywords: CKD, hyperphosphatemia, calcification, phosphate binders.