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American Journal of PharmTech Research

📢 Latest Update: New special issue call for papers on "Emerging Technologies in Research" - Submit by March 31, 2025

📢 Latest Update: New special issue call for papers on "Emerging Technologies in Research" - Submit by March 31, 2025

December 2015 Issue 6

Volume 5, Issue 6 - $2015

Volume 5 Issue 6 Cover

Issue Details:

Volume 5 Issue 6
Published:Invalid Date

Editorial: December 2015 Issue 6

Welcome to the 2015 issue of American Journal of PharmTech Research. This issue showcases the remarkable breadth and depth of contemporary research across multiple disciplines. From cutting-edge applications of machine learning in climate science to the revolutionary potential of quantum computing in drug discovery, our featured articles demonstrate the power of interdisciplinary collaboration in addressing global challenges.

We are particularly excited to present research that bridges traditional academic boundaries, reflecting our journal's commitment to fostering innovation through cross-disciplinary dialogue. The integration of artificial intelligence with environmental science, the application of blockchain technology to supply chain management, and the convergence of urban planning with smart city technologies exemplify the transformative potential of collaborative research.

As we continue to navigate an era of rapid technological advancement and global challenges, the research presented in this issue offers both insights and solutions that will shape our future. We thank our authors, reviewers, and editorial board members for their continued dedication to advancing knowledge and promoting scientific excellence.

Dr Hemangi J Patel
Editor-in-Chief
American Journal of PharmTech Research

Articles in This Issue

Showing 42 of 42 articles
Research PaperID: AJPTR56001

Pelvic Organ Prolapse: Prevalence and Risk Factors

Shaiba Sana Qureshi, Jeetendra Kumar Gupta

The uterus is an organ of the female reproductive system. It is approximately the shape and size of the pear, located inside the pelvis. The bladder, uterus and bowel are supported by a hammock of muscles slung between the coccyx and pubic bone. If these tissues are weakened or damaged, the uterus can slip down in to the vagina which is known as uterine prolpase. It is estimated that the lifetime risk of requiring at least 1 operation to correct incontinence or prolapse is approximately 11%. Common causes of uterine prolpase include obesity, straining on the toilet and hormonal change after menopause. Treatment includes strengthening exercise of pelvic floor muscle. A vaginal pessary for uterine prolapsed during pregnancy was first used in 1949, it can be inserted to support the uterus and reduce the symptoms associated with the prolapsed. Surgery may be needed in many cases.

ProlapseUterine prolapseCystoceleRectoceleVaginal ProlpasePelvic surgery+1 more
200,455 views
60,045 downloads

Contributors:

 Shaiba Sana Qureshi
,
 Jeetendra Kumar Gupta
Research PaperID: AJPTR56002

Protected UV Cabinet for Chemistry Laboratory: A Special Design for Improved Efficiency and Safety

Elahe Keshavarz, Abbas Keshavarz

Laboratory safety is a very important aspect of science. Without it, experimentation could result in very serious injury. Thin Layer Chromatography is very useful to chemists as an analytical technique to separate and identify the compounds in a mixture using chromatograms. In this technique, the chromatograms must be detected under ultraviolet lamp. The design and working of a new unit, protected ultraviolet cabinet, is presented in this paper. This unit can be used for easy viewing and marking of chromatograms and also for safe laboratory.

Safe laboratoryUV cabinetTLC
200,429 views
60,121 downloads

Contributors:

 Elahe Keshavarz
,
 Abbas Keshavarz
Research PaperID: AJPTR56003

Floating with Bioadhesion:A Unique Expansion in Gastroretetive Drug Delivery System

Shaikh Siraj, Molvi K.I, G. J Khan, Shaoor Ahmad, Shaikh Mohsin, Shaikh Salman

Gastro retentive drug delivery system is benefited system in which dosage form retained in stomach prolong period of time and drug release long period of time. Floating system means that float over the surface of the gastric contents when the stomach is full but at the time stomach is empties and the tablet reaches the pylorus the buoyancy of the dosage may be decrease & bioadhesive system becomes dislodged from the stomach wall when the system is full but a floating -bioadhesive system would overcome these drawback of floating and bioadhesive system and improving the therapeutic effect of the drug involved.   So this Floating-bioadhesive dosage forms exhibit a unique combination of floatation and adhesion for prolonged residence in the Stomach. Currently this floating bioadhesive approach is not only used for tablets but also for microsphere, beads & other dosage forms. This review article focus on recently used combination approaches to deliver drug by gastrointestinal drug delivery like Bioadhesive and floating.

Oral drug deliveryfloating with bioadhesiveGastric residence timePolymers etc.
200,337 views
60,221 downloads

Contributors:

 Shaikh Siraj
,
 Molvi K.I
,
 G. J Khan
,
 Shaoor Ahmad
,
 Shaikh Mohsin
,
 Shaikh Salman
Research PaperID: AJPTR56004

In vitro studies of the Ocimum sanctum: Tulsi, Medicinal herb

Deepika Gulati, Priyanka, Minakshi Pal, Nidhi, Ikbal

In the present review on Tulsi, efforts has been done to  congregate the  botanical, and in vitro tissue culture techniques in Ocimum sanctum Linn., a medicinal herb used in the indigenous system of medicine. Tulsi has been adored in almost all ayurvedic literature for its adorable medicinal properties. It is pungent-bitter in taste. Its leaves are hot, light and dry while seeds are considered to be cold in effect. Several medicinal properties of Tulsi are present in the roots, leaves and seeds. It has a wide range of action on the human body mainly ain curing cough, inducer of sweat  and prevents the indigestion and anorexia. Medicinal properties of Tulsi are very well known for thousand years to various parts of the world. In the Indian subcontinent, Hindus considered this medicinal herb as sacred plant.  Exploration the scientific studies of traditional belief of medicinal properties of Tulsi have got acceleration after the middle of the 20th century. Most of the evidences are based upon in-vitro, experimental and a very few are of human studies. Till now, no review present on the in vitro studies of Tulsi, therefore, the present review would discuss the in vitro studies along with its botanical description. This review will definitely help the researchers who deals with Tulsi studies to know its use in proper way, herb appears to be highly valuable due to many pharmacological / medicinal properties.

Ocimum sanctum TulsiMedicinal Herbs
200,385 views
60,200 downloads

Contributors:

 Deepika Gulati
,
 Priyanka
,
 Minakshi Pal
,
 Nidhi
,
 Ikbal
Research PaperID: AJPTR56005

Antihistaminic Activity of Herbal Drug Isolated from Cuscuta reflexa Roxb. Growing in Kashmir Valley

Firdous A Mala, Mushtaq A Sofi, Fayaz A Lone

The aim of present study was to find out the herbal drug which has potential antihistaminic properties. The ethanolic extract of cuscuta reflexa Roxb was found quite useful in showing anti histaminic activities, when tested on experimental albino rats. The rats were divided into four groups of two animals each. Group I was served as control and received water with ad-libitum but not treated and sacrificed for the observation of mast cells which were found 15.50± 2% intact and 88.20±2 % disrupted. In the II group which was treated with ethanolic extract of Cuscuta reflexa, it was noticed that when the dose of 50 mg/kg body weight were given orally with water ,the disruption of mast cells were found 35.60±2 % disrupted and intact mast cells were found 64.40±2 %. In the III group dose of 100 mg/kg body weight, the disruption of mast cells were found 27.70±2 % and intact mast cells were found 72.30±2 %. The rats of group IV received 10mg/kg of prednisolone (reference drug) orally for 4 days.

Mast cell stabilizationCuscuta reflexaPhytochemicalAntihistaminicAsthma.
200,801 views
60,310 downloads

Contributors:

 Firdous A Mala
,
 Mushtaq A Sofi
,
 Fayaz A Lone
Research PaperID: AJPTR56006

Study on Drug Utilization Evaluation of Corticosteroids in a Tertiary Care Teaching Hospital

Pradeep Kumar Thakur, Ayesha Majid, M. Shramik, Shiv Kumar

Steroids are widely prescribed and used by practitioners due to powerful anti-inflammatory and immunosuppressive actions. So, care should be exercised in the rational selection of steroids1. Hence this study was aimed to evaluate the utilization of corticosteroids with the secondary objective to assess co-prescription with corticosteroids, the nature and severity of adverse drug reactions and drug-drug interactions, with an intention to prevent the inappropriate use of corticosteroids. A prospective observational study was carried out in 215 In-patient from various departments of the hospital during 6 months period and subjected to statistical analysis. Out of 215 In-patients, 145 were male and 70 were female, in which most of the patients (22.7%) belongs to age group of 51-60 years. The major complaints were respiratory tract disorders (about 48%) and most of the patients (82%) were prescribed single corticosteroids as monotherapy (42.3%, budesonide) in same or different dosage forms. The commonly used route of administration was inhalation (48%) and is co-prescribed with antibiotics (19.5%). The average number of drugs prescribed was 7.16 drugs per prescription. The mean duration of corticosteroids use was 3.20 ± 1.98 days. The incidence of drug interactions observed were higher (78.6%), with most of them were moderate interactions. The ADR observed with study population were few and minor. The study concluded that, the prescription of corticosteroids is found to be rational except for drug interactions and dosage regimen. Hence the involvement of clinical pharmacists in clinical practice helps to increase proper usage of corticosteroids and optimum outcome.

CorticosteroidsDrug UtilizationDug-Drug InteractionsAdverse Drug Reaction.
200,905 views
60,220 downloads

Contributors:

 Pradeep Kumar Thakur
,
 Ayesha Majid
,
 M. Shramik
,
 Shiv Kumar
Research PaperID: AJPTR56007

Quality-By-Design (QBY) Approach RP-HPLC Method for the Estimation of Nilotinib Hydrochloride and Impurities in Drug Substance

Srikanth Munipalli, Mariappan Soundarapandian, Tulasirao V, V S Prakash Reddy T, Jeenet Jayachandran, Manish Gangrade

The RP-HPLC method development is to define a quality of the product and its process impurities to consistently deliver the intended quality of the drug substance. All the methods which are published are developed through knowledge base, traditional systematic scientific approach. The established method development for Nilotinib hydrochloride was through the vision of “Quality-by-Design” (QbD) approach to estimate the analytical target profile of the target analyte. The robustness of the method has been achieved with an emphasis on establishing the ‘design space’ of the method through the statistical model to ensure the establishment and use of knowledge on the subject. The proposed chromatographic method as Zorbax SB Phenyl (150mmX4.6mmX3.5µm) column thermo stated 45°C using 15 mM potassium dihydrogen phosphate (KH2PO4) with pH-3.5 and Acetonitrile for the gradient elution with the flow rate of 1.0 mL/min. The detection was carried out with the VWD/PDA detector at 240nm. The established design space has been validated against the critical quality attributes (CQA) of the method within the regulatory framework.

Quality-by-Designcritical Quality AttributesDoEDesign Space.
200,870 views
60,372 downloads

Contributors:

 Srikanth Munipalli
,
 Mariappan Soundarapandian
,
 Tulasirao V
,
 V S Prakash Reddy T
,
 Jeenet Jayachandran
,
 Manish Gangrade
Research PaperID: AJPTR56008

Assessment of Health Related Quality of Life in Stroke (Cerebrovascular Accidents) Patients

Sowmya Reddy, Sravanthi Pulipaka, A. Ramesh

To assess the influence pharmacist provided patient education on Health Related Quality of life in patients with cerebro vascular disease. This was a prospective observational and interventional study conducted in neurology department at JSS hospital, Mysore. Morisky medication Adherence Scale (MAS), Validated Stroke Specific Quality of Life Questionnaire (SSQoL), was applied to assess the medication adherence behavior and HRQoL in the enrolled patients. At the end of the study period, 78 patients completed all the study follow-ups and 2 patients were considered as drop outs because of the missed follow-ups. The male population (75%) was three folds predominant than the female population (25%) in the study. Hypertension was observed as the major risk factor for stroke in the study patients. At the end of the study, a statistically significant improvement (P

Health Related quality of lifeStroke Patients.
201,330 views
60,431 downloads

Contributors:

 Sowmya Reddy
,
 Sravanthi Pulipaka
,
 A. Ramesh
Research PaperID: AJPTR56009

Screening of Antibacterial and Antifungal Activity of Canthium coromandelicum from various solvent extracts

S.Sumathi, P.Subha, M.Kavitha Rani, K.Ramalingam, M. Suriyavathana

The present study was aimed to investigate the antibacterial and antifungal activity of aqueous, methanol, ethanol, petroleum ether and acetone extract of canthium coromandelicum using disc diffusion method. The antibacterial activity was detected against Gram-positive and Gram-negative bacteria, Bacillus subtilis, Bacillus ceresu, Staphylococus aureus, Escherichia coli, Klebsiella pneumonia and Pseudomonas aeruginosa. Different solvent extracts of canthium coromandelicum was taken in 100,150,200 and 250 μl/ml. The zones of inhibitions obtained were recorded and analyzed against standard controls, Streptomycin and Amphotericin. The methanolic extract of canthium coromandelicum was showed highest antibacterial activity than ethanolic and aqueous extracts. The higher concentration of methanolic leaves extract of canthium coromandelicum plant, maximum antibacterial activity was observed against to Gram-negative bacteria than Gram-positive bacteria and antifungal activity against to Candida lbicans,  Aspergillus niger. The results indicate that canthium coromandelicum leaves also has potent antibacterial  and antifungal activity.  

Canthium coromandelicumAntibacterialAntifungal activity.
201,378 views
60,312 downloads

Contributors:

 S.Sumathi
,
 P.Subha
,
 M.Kavitha Rani
,
 K.Ramalingam
,
 M. Suriyavathana
Research PaperID: AJPTR56010

Design, Synthesis, Characterization and Biological Evaluation of some novel Heterocyclic derivatives as Anti-Tubercular agents

Ayyadurai Jerad Suresh, Parakkot Ramakrishnan Surya, Antonyraj Velankani

Tuberculosis is a serious threat to public health throughout the world. Schiff bases are compounds carrying the imine or azomethine (–C=N–) functional group. Recent studies report that benzimidazole based Schiff bases possess antibacterial, antimicrobial, anti-tubercular, and anti-inflammatory activities. 1,2 Further it has been known that fluoroaniline moiety can have profound and unexpected results in biological activity 19. A series of benzimidazole and fluroaniline based Schiff bases were designed and docked against crucial mtb enzyme target Glutamine synthetase1. The molecules with good docking-score and multiple interactions were chosen for synthesis. Compounds (BE1, BE2, FA-1, FA-2, and FA-3) were synthesized by reflux condensation reaction with good yield. The newly synthesized compounds were characterized by spectral methods and evaluated for anti- mycobacterial activity against tuberculosis H37RV strain. Anti-tubercular activity was carried out by using Microplate Alamar Blue Assay (MABA) method. The experimental results revealed that Compounds (BE1& BE2) showed promising anti-tubercular activity with an MIC below 0.8 mcg/mL while (FA-1, FA-2, and FA-3) showed moderate anti tubercular activity with an MIC below 6.25 and 12.5 mcg/mL. Key words: Benzimidazole, Schiff base, Fluoroaniline, Docking, Anti-tubercular.

BenzimidazoleSchiff baseFluoroanilineDockingAnti-tubercular.
201,260 views
60,425 downloads

Contributors:

 Ayyadurai Jerad Suresh
,
 Parakkot Ramakrishnan Surya
,
 Antonyraj Velankani
Research PaperID: AJPTR56011

Anti-asthmatic activity of Hydro Alcoholic Extract of Ficus trichopoda Bak. Leaves in Guinea Pig

Randrianavony Patricia, Tsirimaminiaina Hery Lalao Jorosoa, Quansah Nat, Randrianjafitrimo Nicolas, Randimbivololona Fanantenanirainy

The anti-asthmatic activity of Ficus trichopoda was studied in guinea pigs in vivo and in vitro using the isolated guinea pig trachea. 33%, 66% and 100% of the animals treated with the hydroalcoholic extract of Ficus trichopoda at doses 100mg/kg, 200mg/kg and 300mg/kg respectively, were protected from spasm of the air way caused by pulverization of 5% histamine aerosol. In vitro, the isolated guinea pig trachea contracted with histamine at 10-4 M was relaxed with the extract with an EC50 of 65.75µl/ml. The pre-incubation of the isolated trachea in the bath containing the extract at concentrations of 100µl/ml and 200µl/ml increased the EC50 of histamine from (1.10. ± 0.025) 10-6 M to (5.95±0.05) 10-6 M and (1.49±0.01) 10-5M respectively. The maximal contraction provoked by histamine, however, remained unchanged. These effects could be caused by the alkaloids and/or flavonoids present in the extract. Key words: Ficus trichopoda, asthma, guinea pig

Ficus trichopodaasthmaguinea pig
201,485 views
60,376 downloads

Contributors:

 Randrianavony Patricia
,
 Tsirimaminiaina Hery Lalao Jorosoa
,
 Quansah Nat
,
 Randrianjafitrimo Nicolas
,
 Randimbivololona Fanantenanirainy
Research PaperID: AJPTR56012

Effect of Biocontrol agent on Chilli (Capsicum annuum L.) using Seed treatment and Root dipping method

Selvam Gomathi, Varatharaju Ambikapathy, Annamalai Panneerselvam

It has been confirmed that Trichoderma sp. has antagonistic and biological control activity in which T. viride maximum inhibited the pathogenic fungus by the dual culture technique. Seed treatment and application of the antagonist in root dipping are alternative methods for chemical control which could improve the establishment and colonization ability in the plant rhizosphere. This effectiveness of disease control with the added advantage of using small volumes of biocontrol preparations. In seed treatment and root dipping of chilli seedlings the transplanting to the pot after 30th, 60th, 90th and 120th days, the maximum percentage of seed germination and morphometric analysis such as shoot length, root length and yield of fruits were observed in the treatment of T. viride and T. harzianum when compared to control.

Trichoderma spChilliSeed treatmentRoot dipping
201,484 views
60,444 downloads

Contributors:

 Selvam Gomathi
,
 Varatharaju Ambikapathy
,
 Annamalai Panneerselvam
Research PaperID: AJPTR56013

Prevalence of Eating Disorder Gender Wise

Amber Nawab, Mizna Iqbal, Javeria Sheik, Nousheen Shoukat, Misbah Ansari

The conducted study assesses the prevalence of disturbed eating attitude among male and female population in our society and the interrelationship of disturbed eating with a seven basic causes that are responsible for its prevalence is also investigated. This investigation examined the factors, structure and correlates of the reasons of eating disorders. This study is done using 200 sample sizes for each male and female gender, than using a questionnaire, various causes have been evaluated that how much any factor is responsible for the breakthrough of eating attitude abnormality in present days. These results have importance in implicating in view of the high prevalence of eating attitude in individuals. These disorders are due to a complex interaction of social, biological and psychological factors, which bring about such harmful behavior, complex combination of factors can be categorized as genetic, biochemical, emotional, intellectual and environmental.The study reveals that women are more likely than male to develop eating disorder whereas pervasiveness of dieting in women is increased by the value of slimness in western society and the depression.Treatments of abnormal eating attitude always include cognitive-behavioral or group psychotherapy, sometimes along with medication help to resolve it even after few months. The purpose is the evaluation of literature the causes associated with prevalence of eating disorder in society. The important thing is to stop as soon as you recognize these behaviors in yourself then should get help to begin the road to recovery.

eating disorderDepressioncognitive-behavioral and group psychotherapy
201,984 views
60,449 downloads

Contributors:

 Amber Nawab
,
 Mizna Iqbal
,
 Javeria Sheik
,
 Nousheen Shoukat
,
 Misbah Ansari
Research PaperID: AJPTR56014

Health Care Facilities in Karachi’s Hospital

Amber Nawab, Najaf Farooq, Javeria Rahat, Nida Ashraf, Sadia Khalid, Memoona Ashfaq

Health care is the basic need of human being and it is the responsibility of a state to provide all facilities through health care units to improve the health care system and to prevent from diseases. We have done survey in different hospitals and had evaluated the following parameters and calculated the percentages of them in both public and private hospitals:  facility environment (sign boards and accessibility), cleanliness of the premises, management information system, diagnostic services (surgical services, lab services etc.), specialized services (child nutrition services, family planning centre, immunization centre, and diagnostic centre), emergency services, ambulance service and working time of facilities. Our result indicates that Karachi’s health care system is inadequate and inefficient. Most of the private hospitals meet the standard but is expensive and cannot be accessed by poor peoples. In public sector, these facilities are not properly provided and needs improvements. Among them cleanliness and availability of different services like equipment and medicine supply are the major factors which are not satisfactory in public hospitals. These conditions in the health sector may be attributed to a number of factors like poverty like unequal access to health facilities. For equity, efficiency and effectiveness of the health sectors inputs from both the public and private sector is necessary.

Healthfacilitieshospitalsservices
201,661 views
60,630 downloads

Contributors:

 Amber Nawab
,
 Najaf Farooq
,
 Javeria Rahat
,
 Nida Ashraf
,
 Sadia Khalid
,
 Memoona Ashfaq
Research PaperID: AJPTR56015

Burn skin pathogens: Isolation, identification and antimicrobial activity pattern against pyrazole derivatives

Essa Ajmi Alodeani, Mohammad Arshad1* Mohammad Asrar Izhari

Fifty pus samples were collected from burn patients with age ranging 10-55 years with invasive wound infection using sterile cotton swabs. Pure culture were isolated, maintained, preserved and subjected for morphological characterization, accomplished by studying the colony characteristics on different culture plates followed by simple, negative and Gram staining. The cultures then also subjected for biochemical characterization performing various biochemical tests Recovered pathogens were mostly Gram negative. The most common causative agent was found to be Pseudomonas aeruginosa (39.5%), followed by Staphylococcus aureus (25.5%), Acinetobacter baumannii (10%), Klebsiella spp (4.2%), Proteus vulgaris (3.5%) and Escheritia coli (2%). All the previously synthesized compounds were tested on all the isolated pathogens using disc diffusion method and Ciprofloxacin was used as standard drug. The results revealed that out of five, three compounds (1-3) were found to possess significant antimicrobial potential against all pathogens and compounds 4, 5 were also exhibited good activity against E. coli.

Burn skin pathogenspyrazoleantimicrobial activity
201,931 views
60,717 downloads

Contributors:

 Essa Ajmi Alodeani
,
 Mohammad Arshad1* Mohammad Asrar Izhari
Research PaperID: AJPTR56016

Incorporating Design of Experiment (Doe) Principles To Niosomal Cerebroprotective Phytoformulation Development

Shilpa.P.Chaudhari, Jeetendra V Bangar

An cerebroprotective phytoconstituent loaded niosomes for nose to brain delivery was formulated. The main objective of this study was firstly to enhance the solubility and bioavailability of poorly soluble phytoconstituents Rutin and Quercetin, secondly to ease the administration through nasal route and thirdly to investigate the niosome encapsulated phytoconstituent for its cerebroprotective activity. Method: Niosomal formulations were optimized using design of experiment by altering the proportions of range of non-ionic surfactants(Tween 80, Pluronic L81, PluronicP123, span80,captex 200P,Capmul PG NF8,Labrasol), Ratio of cholesterol to surfactant, RPM and sonication. The formulations were prepared by ether injection method. The formulation was then evaluated for morphological characterization, encapsulation efficiency, and viscosity. Cerebroprotective activity of optimized niosomal formulation against bilateral carotid artery occlusion (BCAO) induced stroke in rats was studied. Phytoconstituent niosomes formulated using Tween 80 was found to entrap high amounts of drug, and show sufficient quantity of in vitro release.  Consequences showed that the niosomal formulation of Rutin and Quercetin gives the cerebroprotective action at the dose of 3mg/kg of each drug. Based on the results of histopathology study, it can be concluded that test formulation may have potential to attenuate histopathological alterations caused due to ischemia and can be considered as a promising approach for the cerebroprotective Phytoconstituents.

CerebroprotectiveQuecetinRutinnoisomephytoconstituent
202,240 views
60,628 downloads

Contributors:

 Shilpa.P.Chaudhari
,
 Jeetendra V Bangar
Research PaperID: AJPTR56017

Canvassing Pharmacological Activities Expressed by Hibiscus Sabdariffa Calyx Extracts Using In Vitro Models

Lana Yousif Mutalib

Efforts have been spend for discovering drugs from medicinal plants. Hibiscus sabdariffa plant a well known plant with many traditional uses for treating different aliments have been selected for canvassing pharmacological activities expressed by the different polarity extracts. Results showed significant antidiabetic activity at different concentrations of glucose solution (5, 10 and 20 mM) (p value < 0.001). Significant moderate anti-inflammatory activity comprising in membrane stabilizing activity were expressed by ethanolic extract ranging (29.573±0.709 - 55.040 ±1.659 %) (p value < 0.001) while mild activity were expressed by petroleum ether extract ranging (10.273±0.413 - 22.347± 1.049 %). Approximately similar thrombolytic activity was exhibited by both extracts ranging (56.663±1.3139-21.533±0.656) consider as significant activity in comparison to the control negative (p value < 0.0001). Results conclude canvass the pharmacological activities expressed by different polarity extracts which were showed significant activity in some aspects which may open new venue for production of new drugs derived from nature with less side effects.

Hibiscus sabdariffaMembrane stabilizingThrombolyticIn-vitro anti-diabetic
202,187 views
60,668 downloads

Contributors:

 Lana Yousif Mutalib
Research PaperID: AJPTR56018

Anti-ulcer Activity of Hydro Alcoholic Extract of Acridocarpus excelsus a. Juss Leavesin Rat

Randrianavony Patricia, Raharivelonina Eddie Albertine, Randrianjafitrimo Nicolas, Quansah Nat, Randimbivololona Fanantenanirainy

The anti ulcer activity of the hydro alcoholic extract of the leaves of Acridocarpus excelsus was studied in vivo using WISTAR rats. This study investigated the extract’s effect on gastric ulcer induced by indomethacin orally administered at the dose of 30mg/kg, acid secretion by pylorus ligation and mucus secretion. The results obtained showed that ulceration provoked by the indomethacin on the gastric mucosa surface was reduced from 13.12 ± 0.96 mm2 found inthe control group to 8.15± 0.12 mm2, 2.87 ± 0.41 mm2  and  1.25 ± 0.05 mm2 respectively in the rats treated with the extract at doses 150 mg/kg, 300 mg/kg and 600mg/kg (p

anti-ulcerAcridocarpus excelsusrats.
202,446 views
60,828 downloads

Contributors:

 Randrianavony Patricia
,
 Raharivelonina Eddie Albertine
,
 Randrianjafitrimo Nicolas
,
 Quansah Nat
,
 Randimbivololona Fanantenanirainy
Research PaperID: AJPTR56019

Docking study of Novel Acetamide Derivatives as Specific MAO A Inhibitors

Ashish Pathak, P. K. Singour, Panchanan Gouda, Amit Kumar srivastava4 Sunil Kumar, Anubha Pathak

A Molecular docking study on novel Acetamide derivatives as specific Mono amino oxidase (MAO) A inhibitory agents was performed with a set of 40 compounds to analyze their inhibitory action.  For this, compounds were designed on the basis of available literature and used as Ligands for molecular interaction. The structure of molecular target Mono Amino Oxidase A (MAO A) was retrieved from the PDB database (PDB ID 2Z5X). For comparative analysis Clorgyline, a well-known specific MAO A inhibitor was taken as the standard. Computational docking analysis was performed using PyRx, AutoDock Vina option based on scoring functions. Among 40 compounds the top 11 hits were recognized as promising MAO A inhibitors, according to their docking scores and selected for further study of interaction and visualization. Phenyl sulphanyl derivative with chlorobenzyl amino moiety (Code AD31) showed an optimum binding affinity and stable complex with a molecular target MAO A  with the binding energy of -8.3  kcal/mol as compared to the standard (-7.6 kcal/mol). These results indicated that proposed modification in Acetamide derivatives may produce potent and specific MAO A inhibitors to treat depression with lesser side effects.

DockingMono Amino OxidaseAcetamide derivativesMAO A inhibitorsPyRxAD31.
202,285 views
60,798 downloads

Contributors:

 Ashish Pathak
,
 P. K. Singour
,
 Panchanan Gouda
,
 Amit Kumar srivastava4 Sunil Kumar
,
 Anubha Pathak
Research PaperID: AJPTR56020

Synthesis and Characterization of 3,4-Dihydropyrimidin-2-(1H)-ones: Development of Efficient Protocol for Biginelli Reaction using GeI4

Chandra Mouleswararao Jillepalli, Madhukar Jeripothula

A simple and efficient methodology for the synthesis of 3,4-dihydropyrimidin-2-(1H)-one derivatives has been developed by the condensation of aldehydes, ethyl acetoacetate  and urea using Germanium (IV) iodide as catalyst. All the reactions were carried out at acetonitrile reflux and completed within 2 to 5 hours of reaction with good yields. All the products were very clear and confirmed by spectroscopy analysis.

AldehydesdiketonesureaGeI434-dihydropyrimidinones..
202,651 views
60,900 downloads

Contributors:

 Chandra Mouleswararao Jillepalli
,
 Madhukar Jeripothula
Research PaperID: AJPTR56021

A Community Based Study of Hypertension in Rural Areas of South India

Mohammed Abdul Samee, Nuni Priyanka, Grace M, Binu K. M

Cardiovascular and other non-communicable diseases are currently responsible for two thirds of global mortality. Hypertension is a consistent and independent risk factor for cardiovascular and kidney diseases and stroke. The prevalence of hypertension has increased in urban communities as well as in rural people. There is a strong correlation between changing lifestyle factors and increase in hypertension. Accurate and decisive data about hypertension prevalence and its risk factors is essential for scheming strategies for its effective regulation and prevention. A Community based cross-sectional study was carried out in rural community to monitor prevalence, risk factors, awareness, treatment and control of hypertension in rural population. A total of 627 subjects (males n=369 and females n=258) participated in the study (age > 18 years). The participant’s blood pressure was measured and they were asked to answer a pretested questionnaire. As per the JNC VII report, hypertension was defined. Analysis of data was done using chi square test. The prevalence of hypertension was found to be 49.12% (males: 57.18% and female: 37.50%). About 1/4th of the hypertensive population did not knew about their health status. 60-79 year age group had the highest prevalence of hypertension (49.02%). Bivariate analysis of data was indicative of significant relationship between hypertension with that of age, gender, literacy, body mass index (BMI), physical inactivity, and smoking and alcohol consumption. Hence the prevalence of hypertension was found to be highly prevalent in rural community. We also realized the importance of clinical pharmacist and other health care professional’s involvement in monitoring of health problems reporting possible drug related problems, measuring therapeutic compliance and counselling on lifestyle modification in rural populations.

Cross-sectional studyHypertensionRural CommunityPrevalenceRisk factors.
202,947 views
60,812 downloads

Contributors:

 Mohammed Abdul Samee
,
 Nuni Priyanka
,
 Grace M
,
 Binu K. M
Research PaperID: AJPTR56022

Anti-Cancer Activity of Adhatoda vasica Flowers Against Human Liver Cancer

N.Muruganantham, S.Solomon, M.M.Senthamilselvi

Adhatoda vasica Nees (Acanthaceace) is a well known medicinal plant. Its diverse medicinal activities include cardiovascular protection, abortifacient, antitubercular, antimutagenic, antiulcer, antiasthmatic activities, hepatoprotective, antibacterial and anti cancer activities. It is commonly used in original and traditional folk medicine system. In the prior times, many people used plants to cure diseases just by experimental data without knowing anything about its compounds. A number of evidence has been documented to demonstrate the probability of these plants in pharma industry. In recent years Adhatoda vasica an important medicinal plant that has been extensively used in pharmacological analysis and toxicological studies was found and these phytochemicals were considered to be greater importance in pharma industry. The present study was carried out to evaluate the anticancer activity of the compound isolated from ethyl acetate fractions of Adhatoda vasica and it was found to very good anticancer action against liver cancer. The compound isolated from ethyl acetate fraction of Adhatoda vasica flowers was tested for its anti cancer activity against liver cancer HePG2 cell line by MTT assay. The CTC50 value of sample was 228.5µg/ml against liver cancer HePG2 cell lines. Significant results were observed thereby proving the use of this plant in the traditional system of medicine.

MTT assayanticancer activityAdhatoda vasicaLiver cancer HePG2pharmacological actions etc.
202,729 views
60,814 downloads

Contributors:

 N.Muruganantham
,
 S.Solomon
,
 M.M.Senthamilselvi
Research PaperID: AJPTR56023

Detailed Micromorphological and Pharmacognostic Evaluation of Dadima Fruit (Punica Granatum)

Kunal M Gohil, P. K. Prajapati, C. R. Harisha

Dadima (Pomegranate) is one of the most frequently used drug as a part of medicine and food in Ayurved having varied effects on the human body. Dadima is used in Mukharoga (diseases of mouth), Shukra daurbalya (sexual debility), Samanya daurbalya (general debility), Aruchi (tastelessness), Amlapitta (acidity), etc. and is having effects like Daha shamaka (reduces burning sensation), Jwaraghna (antipyretic), Shukrala (increases semen), Pachana (digestive), Balya (general tonic) and Medhya (brain tonic). Till date the detailed pharmacognostical aspects of Dadima i.e its fruit bark, false septa, seed, powder microscopy including its histochemical evaluation was not found reported systematically. The present study was conducted on the fruit of Dadima (Punica granatum Linn.) for its pharmacognostical characters. The microscopic characters of the fruit show epidermis covered with thick cuticle, single layered epicarp, paranchyma cells filled with prismatic crystals, simple and compound starch grains and oil globules, well developed parenchymatous cells, fibres, schleroids, stone cells, prismatic crystals etc.

DadimaFruitHistochemicalPunica granatumPomegranate. Pharmacognosy.
202,817 views
60,995 downloads

Contributors:

 Kunal M Gohil
,
 P. K. Prajapati
,
 C. R. Harisha
Research PaperID: AJPTR56024

Design and Synthesis of Pyridine-amide Based Compounds Appended Naproxen Moiety as Anti-Microbial and Anti-Inflammatory Agents

Yousry A. Ammar, Eman A. Fayed, Ashraf H. Bayoumi, Marwa A. Saleh, Moustafa E. El-Araby

A series of pyridinamide-containing naproxen derivatives were synthesized through different routes starting with naproxenoyl chloride (2) which, upon reaction with 2-or 3-aminopyridine, gave the corresponding naproxenamide derivatives 3a,b. Also, urea derivatives 5a,b were obtained through the reaction of compound 2 with sodium azide followed by reaction with 2-or 3-aminopyridene via Curtius rearrangement. In addition, naproxenoyl isothiocyanate was synthesized and reacted with 2- or 3-aminopyridine, where the thiourea derivatives 7a,b were obtained. Furthermore, cyanoacetanilide 9 was synthesized and reacted with acetylacetone, arylidenemalononitriles or arylidenecyanoacetate to furnish the corresponding pyridine amide derivatives 10-13. All compounds were screened for anti-inflammatory activity using an in vivo rat paw edema model, from which six of them exhibited higher potency than naproxen. The ulcerogenic effect of most of the active compounds was also screened. In ulcerogenicity screening, the potent anti-inflammatory compounds 3b, 5a and 5b were devoid of any ulcerative effect. This is contrary to naproxen which caused 100% ulcerative effect on all tested animals. Structure-based molecular modeling described that the virtual screening agrees with the SAR of in vivo anti-inflammatory and ulcerogenic activities. Furthermore, all the synthesized compounds were screened for their anti-microbial activity.

NaproxenPyridineAnti-inflammatoryUlcerogenicAntimicrobial activitiesSurflex+3 more
203,334 views
60,863 downloads

Contributors:

 Yousry A. Ammar
,
 Eman A. Fayed
,
 Ashraf H. Bayoumi
,
 Marwa A. Saleh
,
 Moustafa E. El-Araby
Research PaperID: AJPTR56025

Comparitive Evaluation of Three Granulation Processes for Antidiabetic Drug of Gliclazide by Using Design of Experiment

Gangapuram Sushma, Vinay Umesh Rao

In the present study compared and evaluated the three granulation process for controlled release antidiabetic drug of Gliclazide. The effect of three different polymers on the in-vitro dissolution profiles of Gliclazide controlled release tablet was studied. Later combinations of polymers were used and the tablets were prepared by using wet, dry and MADG (Moisture Activated Dry Granulation) process. The study was carried out using full factorial designs of experiments. The release rate of 5 to 6 mg%/ hour was targeted and the effect of the polymers on drug release over 24 hours was evaluated. The DOE experiments have shown that when the combination of polymer is used, the total polymer concentration should be in a narrow range of 32.4% w/w to 37.5% w/w in order to achieve the target dissolution profile for Gliclazide. The effect of three granulation process on the tablet properties and drug release were studied. Wet granulation and MADG process were compared. Finally MADG process is showing better results when compared with other process.

GliclazideMADG (Moisture Activated Dry Granulation)Design of Experiments (DOE).
203,183 views
60,969 downloads

Contributors:

 Gangapuram Sushma
,
 Vinay Umesh Rao
Research PaperID: AJPTR56026

Practical Approach for the Estimation of Alcohol Drug Release from fhe Sustained Release Dosage Forms of Verapamil Hydrochloride

Vagdevi Yerramsetty

The aim of the present study was to develop sustained release formulation of Verapamil Hydrochloride to maintain constant therapeutic levels of the drug for over 12 hrs. Various grades of HPMC polymers, Guar gum and Xanthan gum were employed as polymers. Verapamil Hydrochloride dose was fixed as 120 mg. Total weight of the tablet was considered as 400 mg. Polymers were used in the concentration of 60, 120 and 180 mg concentration. All the formulations were passed various physicochemical evaluation parameters and they were found to be within limits. Whereas from the dissolution studies it was evident that the formulation (F6) showed better and desired drug release pattern i.e., 96.10 % in  12 hours containing Guar gum polymer in the concentration of 180mg . It followed zero order release kinetics. For the optimized formulation alcohol effect has been studied by using various concentrations of alcohol in dissolution medium. As the concentration of alcohol increases the sustained action of polymer was decreased. Hence it was concluded that alcohol has significant effect on drug release pattern.

Verapamil HydrochlorideHPMCGuar gumXanthum gum.
203,210 views
60,970 downloads

Contributors:

 Vagdevi Yerramsetty
Research PaperID: AJPTR56027

Isoniazide Sustained Released Tablet by Using Various Hydrophilic Polymers Formulation and In-Vitro Evaluation

Ramesh Salugu

The aim of the present study was to develop sustained release formulation of Isoniazide to maintain constant therapeutic levels of the drug for over 12 hrs. Various polymers such as Guar gum,HPMCK100 M,PEG 6000 AND Carbopol 934 p were employed as polymers. Isoniazide dose was fixed as 100 mg. Total weight of the tablet was considered as 400 mg. Polymers were used in the concentration of 100, 150 and 200 mg concentration. All the formulations were passed various physicochemical evaluation parameters and they were found to be within limits. Whereas from the dissolution studies it was evident that the formulation (F6) showed better and desired drug release pattern i.e.,96.10 % in 12 hours. It followed zero order release kinetics mechanism.

IsoniazideGuar gumHPMCPEG 6000 and sustained release tablets.
203,666 views
61,074 downloads

Contributors:

 Ramesh Salugu
Research PaperID: AJPTR56028

Compaction and Compressibility Characteristics of Modified Starches Derived from Plectranthus Esculentus by Direct Compression

Khalid GM, Musa H, Olowosulu AK

In this study, the compactability and compressibility of modified starches obtained from the tuber Plectranthus esculentus were evaluated in comparison with the standard microcrystalline cellulose (MCC 101). Starch was extracted from the fresh tubers of P. esculentus by wet milling. Three modifications of the starch were made, acid hydrolysis (APS), pregelatinization (PPS), and ethanol dehydrated pregelatinization (PPE). Tablet’s compaction characteristics were studied using models of Heckel and Kawakita equations, while compressibility index were evaluated using tablet’s density measurements. The percentage recovery yields for the three modifications revealed that PPS has the highest yield in comparison to PPE and APS. Compaction and compressibility evaluation demonstrate that modification by acid hydrolysis (APS) and ethanol dehydration of the pregelatinized starch (PPE) produced directly compressible filler/binder that can plastically deform on compression base on their mean yield pressure Py and the inverse measure of plastic deformation Pk values were lower in comparison to the pregelatinized modification (PPS). Compressibility index also revealed that APS and PPE are more compressible and better comparable to mcc 101 than PPS.

Compactioncompressionstarchmicrocrystalline celluloseHeckel and Kawakita equations.
203,661 views
61,023 downloads

Contributors:

 Khalid GM
,
 Musa H
,
 Olowosulu AK
Research PaperID: AJPTR56029

Compatibility Study and Solubility Enhancement of Febuxostat Using Box Behnken Design

Sahaj Neupane, Uttam Budhathoki, Ashwinee Kumar Shrestha

Compatibility study (based on microenvironmental pH and isothermal stress testing) of Febuxostat was carried out with selected excipients (Microcrystalline cellulose (MCC), Mannitol, Lactose, Hydroxypropyl-β-cyclodextrin (HP-β-CD), Polyethylene glycol(PEG) 6000, Polyvinyl pyrollidoneK30, Eudragit EPO, Sodium starch glycollate, Croscarmellose sodium (CCMC), Sodium lauryl sulphate, Magnesium stearate (MgS), Sodium steryl fumarate, Aerosil 200 and Purified talc) using FTIR and HPLC. Among them, Aerosil 200, MgS, Purified talc, Lactose and MCC were selected for the formulation of Febuxostat tablet. Three different polymers viz. PEG 6000, HP-b-CD and CCMC were selected as independent variables to enhance the dissolution rate by their complexation. Fifteen formulations obtained from Box Behnken design (BBD) (Minitab 16) were prepared through kneading method. Contour plot suggested CCMC (13.01mg) and HP-b-CD (65.45mg) excluding PEG 6000 for optimized formulation. Drug release profile of optimized formulation compared separately with formulation without filler, without polymer, physical mixture and a marketed product using similarity (fs) and dissimilarity (fd) factors showed similarity with marketed product. Similarly, similarity and dissimilarity factors for formulation without filler and optimized formulation was obtained within the range (fs= 82.34 and fd=5.42) indicating that the filler does not have any effect on the drug release. fs and fd for formulation without polymer and physical mixture lied outside the range suggesting the importance of the polymer complexation in the formulation. An accurate, simple, precise and robust reversed-phase liquid chromatographic method was developed for the estimation of Febuxostat. Furthermore, solid state characterization evaluated by FTIR showed that complexation between the polymers has occurred in the optimized formulation.

Compatibility StudySolubility EnhancementFebuxostatBox Behnken DesignComplexation
203,551 views
61,154 downloads

Contributors:

 Sahaj Neupane
,
 Uttam Budhathoki
,
 Ashwinee Kumar Shrestha
Research PaperID: AJPTR56031

Physico-Chemical Investigation of Quality of Drinking Water Affected by Ganapati Visarjan in Maharashtra, India

S.R. Kondulkar, R.S. Shekar, A.K. Wanjari, C.N. Surjuse

The object of this work was to study the quality of drinking water from rivers in selected area of Amravati district of Maharashtra, India.  This paper is intended to be a study concerning with surface water quality in various river affected by Ganapati Visarjan. While monitoring samples were collected in September 2015 from sampling sites before Ganapati Visarjan and after Ganapati Visarjan to evaluate relative differences in Physicochemical properties of river water such as Turbidity, Silica, Hardness, COD, TDS, DO, Phosphate, Alkalinity, Sulphate, Ca2+, Mg2+, Cl-, etc. The results are compared with standards of World Health Organisation (WHO), United States Public Drinking water Standard (USPH) and Indian Council of Medical Research (ICMR).

Physico-chemical analysis of waterHardnessCODBODMaharashtraganapati Visarjan.
203,958 views
61,231 downloads

Contributors:

 S.R. Kondulkar
,
 R.S. Shekar
,
 A.K. Wanjari
,
 C.N. Surjuse
Research PaperID: AJPTR56032

UV-AUC Method Development and Validation for Estimation of 1h, 1’-H-2, 2’-Bibenzimidazole Impurity in Telmisartan Bulk and Formulation

Poonam P. Patil, Veena S. Kasture, K. Vanitha Prakash

The aim of present work was to develop an accurate, precise, reproducible and economical UV spectrophotometric method for estimation of 1H, 1’-H-2, 2’-Bibenzimidazole Impurity In Telmisartan Bulk and Formulation. This method was based on area under curve of UV spectrum between 235 to 254 nm and validated as per ICH guideline Q2 (R1). The method has followed linearity in the range of 5-30μg/ml. The value of correlation coefficient was 0.998. Satisfactory values of Percent relative standard deviation for the intra-day and inter-day precision indicated that method is precise. Results of the recovery studies (97.63% to 98.66 %) showed accuracy of the method. LOD and LOQ were calculated as 0.3221μg/ml and 0.9761 μg/ml, respectively. The developed method can be used for routine estimation of 1H, 1’-H-2, 2’-Bibenzimidazole Impurity In Telmisartan Bulk and Formulation.

TelmisartanUV spectrophotometryArea under curveValidationImpurity
204,131 views
61,240 downloads

Contributors:

 Poonam P. Patil
,
 Veena S. Kasture
,
 K. Vanitha Prakash
Research PaperID: AJPTR56033

Antimicrobial and Phytochemical Analyses of Bioactive Compounds of Butea monosperma (Lam.) Taub. and Butea superba Roxb. from Jharkhand

Raphael R Marandi, S. John Britto, Kindo Ignace

Butea monosperma and Butea superba belonging to the family, Fabaceae are most exploited medicinal plants by different tribal groups of Jharkhand. They are commonly found in the hills and jungles of Jharkhand and are used against arthritis, osteoarthritis, diarrhoea, dysentery, snakebite, male sexual debilities, sunstroke, leucorrhoea, anthelmintic and filariasis. Ethanolic exacts of the barks and flowers of both the plants did not exhibit significant antibacterial and antifungal activities. Phytochemical analyses revealed a total of 14 bioactive compounds from the barks and flowers of B. monosperma and B. superba.  Successful management of several diseases among the ethnic groups of Jharkhand, is indicative of presence of curative drugs without toxicity and side effects, and it could further the isolation and purification of active compounds contained in them.

Butea monospermaButea superbaPhytochemicalsHPLCGC-MSAntibacterial+3 more
203,896 views
61,331 downloads

Contributors:

 Raphael R Marandi
,
 S. John Britto
,
 Kindo Ignace
Research PaperID: AJPTR56034

A New Stability Indicating RP-HPLC Method for Simultaneous Determination of ARTEMETHER And Lumifantrine in its Bulk and Pharmaceutical Dosage Forms

Prathyusha Vilamburu

The chromatographic conditions were successfully developed for the separation of by artemether and lumifantrine using  symmetry C18 column (4.6×150mm) 5µ,  A wavelength of 290 nm was selected and the mobile phase consists of triehtylamine  buffer (PH 6 adjusted with ortho phosphoric acid) and methanol in 20:80 % v/v ratios at a flow rate of 0.8 ml/min were found to be optimum conditions for analysis. The peaks were well resolved with symmetry C18 column. System suitability studies were also carried out which includes theoretical plates, resolution and tailing factors etc. The accuracy studies were shown as % recovery for Artemether and Lumefantrine at 50 %, 100 % and 150 %. The limits of % recovered should be in the range of 98-102 %. The results obtained for Artemether and Lumefantrine were found to be within the limits. Hence the method was found to be accurate. The accuracy studies showing % recovery of Artemether were found to be 99.9 %, 99 % and 99.9. % respectively and the % recovery of Lumefantrine were found to be 99.9%, 99.9 % and 98.9 % respectively. In the System precision study, the % RSD was found to be less than 2 %. For Artemether 1.7 and Lumefantrine 1.2 which indicates that the system has good reproducibility. For ID precision studies six replicate injections of Artemether and Lumefantrine were performed. The % RSD was determined for peak areas of Artemether and Lumefantrine. The acceptance limit should not be more than 2 % and the results were found to be within the acceptance limits. Using the optimized chromatographic conditions, chromatograms of mixed standard solutions which contained Artemether and Lumefantrine were recorded. Retention times of Artemether and Lumefantrine were found to be 2.9 min and 4.6 min respectively. Calibration curves were obtained by using peak area vs. concentration. Artemether and Lumefantrine show linearity in the range of 0.1 – 0.5 µg/ml and 0.6 – 3.0 µ/ml. Calibration curve was plotted and correlation co-efficient for both the drugs Artemether and Lumefantrine were found to be 0.999 and 0.999 respectively

StablitityHPLC
204,295 views
61,346 downloads

Contributors:

 Prathyusha Vilamburu
Research PaperID: AJPTR56035

Synthesis and Characterization of 1,4-Dihydropyridines Catalyzed by Zn(OTf)2

D. Sumalatha, G.S.S.N.Reddy, Y. Venkateswarlu, L. N. Sharada, B. Nagaiah, A. Venkat Narsaiah

A simple and efficient protocol for the synthesis of 1,4-dihydropyridines has been developed. In this one-pot multicomponent condensation methodology, all the reactants aldehydes, β-ketoester, ammonium acetate and catalyst were subjected to react at acetonitrile reflux to afford the corresponding products in very good yields. In all reactions, the catalyst Zn(OTf)2 was used in catalytic amount only and all the products were characterized by their spectroscopy analysis.  

AldehydesdiketonesNH4OAcZn(OTf)214-dihydropyridines.
204,150 views
61,308 downloads

Contributors:

 D. Sumalatha
,
 G.S.S.N.Reddy
,
 Y. Venkateswarlu
,
 L. N. Sharada
,
 B. Nagaiah
,
 A. Venkat Narsaiah
Research PaperID: AJPTR56036

Formulation and Evaluation of Self Emulsifying Emulsion of Ketaconazole

Shashank Khugshal, Ashutosh Badola, Preeti Kothiyal, Shweta Baluni

The aim of present work was to develop a self emulsifying emulsion of ketaconazole for the topical delivery system which is useful in the treatment of fungal infection. Prepared SEDDS was investigated for different parameters. All the prepared SEDDS showed acceptable physical properties concerning colour, viscosity, melting point etc. The results of in -vitro drug release showed that carbopol 934 was the formula of choice as it showed better drug release & antifungal activity. The percentage cumulative drug released was determined by UV spectrophotometer. FTIR studies revealed that drug and all excipients are compatible. The data obtained from in- vitro permeation studies was treated by various conventional mathematical models (zero order, first order, Higuchi and Korsmeyer- peppa’s) to determine the release mechanism from the designed self emulsifying formulations. Selection of a suitable release model was based on the values of R2 (correlation coefficient), k (release constant) obtained from the curve fitting of release data. It was found that all the formulations follows the first order kinetics. The regression coefficients for the all formulations F1 to F4 of Higuchi plot was found to be almost linear. Key words: SEDDS, ketaconazole, antifungal drug, topical drug delivery.

SEDDSketaconazoleantifungal drugtopical drug delivery.
204,453 views
61,376 downloads

Contributors:

 Shashank Khugshal
,
 Ashutosh Badola
,
 Preeti Kothiyal
,
 Shweta Baluni
Research PaperID: AJPTR56037

Formulation and Evaluation of Tioconazole Emulgel for Topical Drug Delivery System

Shailendra Panwar, Sayantan Mukhopadhyay, Preeti Kothiyal

The aim of present work was to develop a emulgel for the topical delivery system which is useful in the treatment of vaginal fungal infection. Emulgels having advantage of both emulsion & gels which act as a controlled drug delivery system for topically applied drugs. The Gel in formulations were prepared by dispersing Carbopol 934 & Carbapole 940 in purified water. Then oil phase & aqueous phase of the emulsion were prepared. Both the oily and aqueous phases were heated separately & then oily phase were added to the aqueous phase. add Glutaraldehyde in during of mixing of gel and emulsion in ratio 1:1 to obtain the emulgel. Prepared emulgels was investigated for different parameters. All the prepared emulgels showed acceptable physical properties concerning colour, viscosity, melting point, pH value, and spreadability. The results of in-vitro drug release showed that carbopol 934 was the formula of choice as it showed better drug release & antifungal activity. FTIR studies revealed that drug and all excipients are compatible. The data obtained from in- vitro permeation studies was treated by various conventional mathematical models (zero order, first order, Higuchi and Korsmeyer- peppa’s) to determine the release mechanism from the designed emulgel formulations. Selection of a suitable release model was based on the values of R2 (correlation coefficient), k (release constant) obtained from the curve fitting of release data. It was found that all the formulations follows the first order kinetics. The regression coefficients for the all formulations F1 to F4 of Higuchi plot was found to be almost linear. Key words: Emulgel, tioconazole, antifungal drug, topical drug delivery.

Emulgeltioconazoleantifungal drugtopical drug delivery.
204,746 views
61,418 downloads

Contributors:

 Shailendra Panwar
,
 Sayantan Mukhopadhyay
,
 Preeti Kothiyal
Research PaperID: AJPTR56038

Seasonal variation of groundwater quality of Akola city, Maharashtra, India

Atulkumar B. Sahare

The Akola city, Maharashtra, India, is selected to study the seasonal variation of groundwater quality, where the groundwater is the main source for household and drinking purposes. Groundwater samples collected seasonally in summer, Monsoon and winter, during the years 2013 from ten bore wells and analysed for pH, EC, TDS, DO, TA, TH, Ca++, Mg++, Na+, K+, Clˉ, NO3ˉ and SO4ˉ ˉ. The ground waters mainly belong to carbonated alkali type and are controlled by evaporation dominance, due to the influence of tropical savannah climate and anthropogenic activities. A comparison of the groundwater quality in relation to drinking water quality standards proves that most of the water samples are not suitable for drinking purposes, especially in pre-monsoon period. These conditions are caused due to leaching of salts from the overlying materials by infiltrating recharge waters.

Groundwater qualityAkola citySeasonal variationhard water.
204,660 views
61,510 downloads

Contributors:

 Atulkumar B. Sahare
Research PaperID: AJPTR56039

A UV Spectrophotometric Method for the Determination of Methotrexate in Pharmaceutical Dosage Form

Maroti B. Shelke, Faijal E. Patel, Shivani U. Gadve, Pratiksha B. Shinde, Mukesh T. Mohite

Two simple, precise and economical UV methods have been developed for estimation of Methotrexate in bulk formulation. Method A involves measurement of UV absorbance in Zeroorder derivative at 303nm. Method B deals with area under curve measurement (AUC method), which involves the calculation of integrated value of absorbance with respect to wavelength between 300-306nm.  The drug follows Beer-Lambert’s law in the concentration range of 3-15μg/ml in both the methods. Results of analysis were validated statistically and were found to be satisfactory. Thus proposed methods can be successfully applied for estimation of Methotrexatein routine analytical work.

MethotrexateZero order derivativesArea under Curve method (AUC)UV Spectrophotometer.
205,022 views
61,498 downloads

Contributors:

 Maroti B. Shelke
,
 Faijal E. Patel
,
 Shivani U. Gadve
,
 Pratiksha B. Shinde
,
 Mukesh T. Mohite
Research PaperID: AJPTR56040

Antioxidant Activities of Some Selected Wild Edible Plants of Arunachal Pradesh State In India and Effect of Solvent Extraction System

Tapan Seal, Kaushik Chaudhuri

The aim of present study was to evaluate the antioxidant activities of four different solvent extracts of four wild leafy vegetables e.g. Solanum nigrum  Phytolacca esculenta, Piper pedicellatum and Pouzolzia hirta  and fruits of Melodinus khasianus collected from Arunachal Pradesh  state in India. The extracts of the plants were examined for their antioxidant activities by using free radical 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging method, ABTS radical scavenging ability, reducing power capacity, estimation of total phenolic content, flavonoid content and flavonol content.. The result showed that the total phenolics, flavonoids and flavonols of the different extracts of the investigated samples ranged from 7.49 ±3.55 - 305.38 ±2.78 mg gallic acid equivalents (GAE)/g dry extract, 4.13±0.02 - 171.01±0.99 mg rutin equivalent /g dry extract and 18.90±0.15 to 326.21±1.84 mg quercetin equivalent /g dry extract respectively. Furthermore the plant extracts exhibited good free radical scavenging capacity. The solvent used to extract the active constituents from plants were benzene, chloroform, acetone and methanol. The different levels of antioxidant activities were found in the solvent systems used. The results indicate that these wild edible vegetables could be utilized as natural antioxidant.

Antioxidant activityDifferent solvent extractsArunachal PradeshWild edible plants
204,924 views
61,481 downloads

Contributors:

 Tapan Seal
,
 Kaushik Chaudhuri
Research PaperID: AJPTR56041

A Novel Ophthalmic Pharmaceutical Nano emulsion: Methods of Preparations, Characterizations, and Applications

Riad K. El Qidra

Ocular delivery drug is the major challenge faced by formulation scientists and pharmacologists because of the poor ophthalmic bioavailability. One of the most promising technologies is the Nanoemulsion drug delivery system. Nanoemulsions have the potential advantages in pharmaceutical industries because of the transparency at high droplet volume fraction, safe, patient compliant formulation which is being applied to enhance the solubility and higher rate of  bioavailability of lipophilic drugs, ease of manufacturing and permeation over conventional formulations that convert them to important drug delivery systems. As the drug is lipophilic in nature it can be easily soluble into the oil phase that is used in the formulation of the nanoemulsions and also reduced particle size to the nanometer range favors the formulation to achieve more surface area there by solubility of the lipophilic drug can be achieved. Nanoemulsions are submicron sized emulsion that is under extensive investigation as drug carriers for improving the delivery of therapeutic agents. These are clear, thermodynamically stable, isotropic liquid mixtures of oil, water, surfactant and co-surfactant. These are oil-in-water (o/w) type of emulsions with the average droplet size ranging from 5 - 200 nm and shows a narrow size distribution. Reduction in droplet size to nanoscale leads to change in physical properties such as optical transparency & unusual elastic behavior. Nanoemulsions have future widespread applications in different fields such as pharmaceutics, food technology, diagnostics drug therapies and biotechnologies. This review mainly discussed about the importance of ophthalmic nanoemulsions over other dosage forms, advantage and disadvantage various methods of preparation, characterization of nanoemulsions and applications.

OpthalmicNano Emulsion
204,952 views
61,562 downloads

Contributors:

 Riad K. El Qidra
Research PaperID: AJPTR56042

Gastro-Protective Potential of Trichosanthes Dioica in Experimental Animals

Devansh Mehta, Anuj Kumar Sharma

Ulcer is tissue erosion, either on skin or inside the body, for e.g. lining of gastrointestinal tract. The diverse causes for ulcer development range from, Genetic factors to Physiological/ Environmental factors. Helicobacter pylori recently found to be responsible for majority of peptic ulcer cases. Peptic ulcer diseases are extremely common in developing and developed countries with annual incidence of 1 to 3 per 1000th of the population. PUD is an important cause of morbidity and health care costs; estimates of expenditures related to work loss, hospitalization, and outpatient care are $5.65 billion per year in US. It affects 1 in 10 men & 1 in 15 women in Europe. Due to side effects from conventional drug therapy and non-specificity of therapeutic choices, there is inclination towards Traditional medicines with low cost and low adverse effect incidences. Many photochemical substances have been found to be effective against pathogenic factors responsible for ulcer formation and its severity. Alkaloids, Flavonoids, Terpenoids, & Tannins have been researched to possess potent Gastroprotective activities. Glycosidal components have been found effective against Helicobacter pylori gram negative strains. Trichosanthes dioica (Roxb.) found in flora and fauna of Uttar Pradesh have high concentrations of terpenoids, flavonoids, cucurbitacins, which have been researched for having anti-inflammatory and wound healing activities. The present study clearly shows the effectiveness of Trichosanthes dioica (Roxb.) in having Gastroprotective activity. This gives clear indication that Trichosanthes dioica shall be researched upon to bring effective drug in market against ulcer diseases.

Trichosanthes dioicaPeptic Ulcer Diseases (PUD)Gastroprotective potentialAnti-ulcer drugsTrichosanthes speciesMethonolic extract of Trichosanthes dioica (MeTD)
205,394 views
61,638 downloads

Contributors:

 Devansh Mehta
,
 Anuj Kumar Sharma
Research PaperID: AJPTR56043

Synthesis, QSAR and Antibacterial Activity of Some Novel Azetidinone Containing Quinoline Derivatives

M. Nagendra Babu, N. Pramod, P. Amrutha, D. Kanchana, G.Mahaboob Basha, C. Gopinath

The synthesis of novel azetidinone containing quinoline derivatives involves, cyclization reaction to form quinoline-3-carbaldehyde followed by Schiff’s base formation and finally resulted into azetidinone derivatives as target molecules. Firstly, quinoline-3-carbaldehyde was prepared from N-phenylacetamide with POCl3 in dimethyl formamide (Vilsmeyer Hacck reagent) under reflux through cyclization. The formed quinoline derivative was treated with substituted aromatic amines in presence of glacial acetic acid through grindstone process to get Schiff’s bases of quinoline derivatives. Finally, these Schiff’s bases were allowed to react with acetyl chloride and triethyl amine in presence of dimethyl formamide under reflux to achieve azetidinone ring formation resulting in the novel azetidinone containing quinoline derivatives. The synthesized compounds were identified by melting point and TLC as well as characterized by IR spectroscopy. The titled compounds were screened for antibacterial activity using agar well diffusion method against Staphylococcus aureus, Escherichia coli. Most of the compounds showed good antibacterial activity against G(-)ve bacteria and mild activity against G(+)ve bacteria when compared to the standard, Amoxicillin. QSAR study was performed, results revealed that the target molecules possess higher logP values which indicates that the physicochemical parameters proportional to the biological activity. Due to its high logP value the target molecules may possess CNS activities.

ilsmeyer Hacck reagentquinoline derivativesazetidinonesantibacterial activities.
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Contributors:

 M. Nagendra Babu
,
 N. Pramod
,
 P. Amrutha
,
 D. Kanchana
,
 G.Mahaboob Basha
,
 C. Gopinath
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