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Fifteen bacterial isolates were purified from Wady El Natrun loam salty calcareous sand soil textures and subjected to screen for their ability to produce folic acid. Bacterial isolate number WN12 was highly producer  of folic acid (Vitamin 9) and it was selected tentatively to be identified physiologically and phylogeny. The strain grows at 15-50°C, pH 6.0-12.5 and at salinities of 3.0-27.5 % NaCl (w/v). It was Gram positive , translucent brownish smooth color colony, long rods; non spore forming, motile by peritrichous numerous flagella.WN12 isolate identify as Bacillus alcalophilus subsp halodurans .The TLC Rf values for crude folic acid was 0.7 by using solvent system n-butanol, glacial acetic acid, water, methanol (20:10:20:5 v/v). The UV analysis of the fractions to the purified bacterial folic acid peak at 270 nm .Also, TEM images estimated the average size nearly around 0.1µm. While HPLC chromatograms of free folic acid maximum absorption wavelength was selected at 280 nm .And the FTIR spectra show absorption bands for pure folic acid infrared spectrum showed several identical absorption bands including OH, band at 3500 cm−1, C-H, band at 2900 cm−1 C=O band at 1100 cm−1. Comparison of the two synthesis conjugates Nano gold (AuNPs) and Nano silver(AgNPs) with folic acid (FA) characterization were  done . Six treatment were tested for measuring  infected dose 50 (IC50)  on four cancer cell lines,. Cytotoxic effect of  folic acid alone and Ag NP showed a variable pattern of reactivity to cell lines arranged ion in the order of MCF7> HCT> CaCO2> HEPG2 (P CacO2 >HCT >HEPG2 (P