V. Anitha

A simple, specific, accurate and precise reverse phase high performance liquid chromatographic method was developed and validated for the estimation of Venlafaxine hydrochloride in pure and Pharmaceutical dosage form. Kromasil C18 column having 150 mm x 4.6 mm internal diameter, 5 µm particle sizes in isocratic mode with mobile phase containing mixture of methanol and water in the ratio of 65:35 v/v was used. The flow rate was 1.0 ml/min and effluents were monitored at 225 nm. The retention time for Venlafaxine was 2.424 min. The method was validated for linearity, accuracy, precision, specificity, limit of detection, limit of quantification and robustness. Limit of detection and limit of quantification were found 2.97µg/ml and 9.92 µg/ml respectively and recovery of Venlafaxine from tablet formulation was found 100.4 %. The proposed method was successfully applied for the quantitative determination of Venlafaxine in tablet dosage form.