Tamizhmani

Mosquitoes are a major vector for diseases such as malaria, dengue, and yellow fever. Mosquito repellents today contain many synthetic chemicals, such as DEET, picardin, and permethrin. To safely repel mosquitoes, less effective alternatives made from essential oils have been used. To try to increase the effectiveness of the essential oil repellents, tests were conducted to find out if the essential oils have increased effectiveness when used in conjunction to each other. It was hypothesized that the essential oil mixture containing lemon eucalyptus and rosemary would make the most effective mosquito repellent. To test the hypothesis, a custom-made mosquito testing chamber with an aquarium warmer was used. A sugar based attractor and the prospective repellent solution were placed on a piece of cloth, which was tied to the aquarium warmer. The aquarium warmer was then placed into the testing chamber, where the mosquitoes resided. The effectiveness of the mosquito repellent was determined by the number of landings on the aquarium warmer over time. The results showed that the mosquito repellent made from Bael and eucalyptus was the most effective mosquito repellent.

Clobazam is an antiepileptic drug. There have been very less number of analytical methods developed for estimation of Clobazam in pure bulk form and in dosage form. In the present research a simple, accurate, precise and cost effective High performance liquid chromatographic method for the estimation of Clobazam, in bulk and pharmaceutical dosage form was carried out. HPLC method was developed on a Symmetry C-8 (4.6×150mm), 3.5µm particle, reversed-phase column. The mobile phase was acetonitrile: phosphate buffer (0.05M, pH- 4.5), 60:40 (v/v) at a flow rate of 0.8ml/min. The eluate was monitored at 231 nm. The method was validated reaching satisfactory results for selectivity, precision and accuracy. The retention time of the drug was found to be 3.38min in the mobile phase, acetonitrile: 0.05M potassium dihydrogen orthophosphate buffer (pH-4.5) 40: 60 (v/v). A linear response was observed in the range of 20-60µg/ml with a regression coefficient of 0.999. Validation parameters were carried out as per the guidelines of International Conference for Harmonization (ICH). This method can be used in the industries for determination of Clobazam to analyze the quality of formulation without interference of the excipients.