Tamizh Mani .T

A simple, specific, accurate, precise and sensitive RP- HPLC method has been developed for the rapid estimation of Ganciclovir in bulk and its formulations. The chromatographic separation was carried on Grace smart RP-18 column (250 x 4.6 mm, 5 μm), using Methanol: Citrate buffer (0.05M) at PH-5.2 with KOH 70:30 (v/v) as mobile phase, at a flow rate of 1.0 ml/min.  The detection was carried out at 254 nm and drug eluted with a retention time of 2.982 min. Beer’s law was obeyed in the concentration range of 10-60μg/ml with correlation coefficient 0.999. The method has been validated according to ICH guidelines for specificity, linearity, accuracy, precision, robustness, ruggedness, LOD and LOQ. The method was found to be specific, accurate, and precise, robust, rugged and sensitive. The developed method was good linearity, novel, rapid for the estimation of Ganciclovir in bulk and capsule dosage form. Thus it can be employed for the routine analysis.

An accurate, simple, rapid, precise and economical RP- HPLC method has been developed for the rapid estimation of ImatinibMesylate in bulk and pharmaceutical formulation. The separation was achieved on Phenomenex C18 G column ( 250 x 4.6 mm i.d, 5 μm), using Methanol : 1-octanesulphonic acid (0.05M) at PH-8 with KOH  70:30 (v/v) as mobile phase, at a flow rate of 1.0 ml/min. Detection was carried out at 269 nm and drug eluted with a retention time of 5.548 min. Beer’s law was obeyed in the concentration range of 2-12μg/ml with correlation coefficient 0.999. The method had been validated according to ICH guide lines for specificity, linearity, accuracy, precision, robustness, ruggedness, LOD and LOQ. The method was found to be specific, accurate, and precise, robust, rugged and sensitive. The proposed method was convenient for quantitative routine analysis and quality control of ImatinibMesylate in bulk and pharmaceutical dosage form. Key words: Imatinib Mesylate , RP-HPLC, Validation, 1-Octanesulphonic acid.