Sulaiman Kamal Al Habib

This study examines the effects of lactoferrin and black seed oil on microorganisms, as well as the extent to which they inhibit the growth of bacteria. Resistance to antibiotics has been outlined in previous research; therefore, a new strategy is required for sourcing natural biological materials for the extraction of antimicrobial peptides (AMPs). This new strategy should enable microbe efficacy, thus providing a new and innovative method for combatting microbes. Lactoferrin from humans and cows has undergone rigorous analysis though camel lactoferrin (CLF), but this analysis has also gone largely underreported. The current research study addresses CLF by evaluating its mechanisms of antimicrobial activity. This activity was observed in the contexts of Staphylococcus aureus (S. aureus), Streptococcus agalactiae, Streptococcus pneumonia, Streptococcus pyogenes and Candida albicans. The primary research aim of this study is to determine the mechanisms and properties of lactoferrin and black seed oil in the contexts of the five bacteria groups mentioned above. The materials used in the analysis included ethanol, sabouraud dextrose, agar, nutrient agar and filter paper discs (which were used for the AMP agent in sterile conditions—the disc diffusion method). AMPs, native and mutant (camel) lactoferrin peptides, nigella sative (NS) oil and Nigella sativa oil (black seeds) were also used. The microorganisms were synthesized from the lab. It has been found that lactoferrin is effective in bacterial applications. Gram-positive bacteria has also been found to be more efficient when the synergistic action of camel lactoferrin (SAoCL) mutant peptide is used with NS oil. This study shows that lactoferrin has promising results in the binding of iron in microbe growth prevention. Additionally, observations of SAoCL, the mutant peptide, show that SAoCL, alongside NS oil, hinders the further development of bacteria.