Ramachandran D

This paper portrays the advancement and approval of a solidness showing fluid chromatographic strategy for measure of desvenlafaxine in unadulterated and in plans utilizing X-Terra RP C18, 250x4.6 mm, 5 µm particle size column with detection at wavelength 226nm. In the present investigation the mobile phase comprising of sodium dihydrogen orthophosphate buffer (pH 4.0) and acetonitrile in the proportion of 60:40%/v with the Flow rate of 1.0ml/min and column temperature at surrounding temperature uncovered the better resolution and affectability for desvenlafaxine. The maintenance time of desvenlafaxine peak was about 3.632min independently. The created approach be accepted according to ICH regulations which incorporate framework appropriateness, particularity, linearity, exactness, accuracy, vigor, toughness, affectability, cutoff of detection and measurement contemplates and the outcomes were incorporated in this paper. Keywords: Desvenlafaxine, stability indicating method, RP-HPLC, ICH regulations.

An isocratic reverse phase liquid chromatography (RP-LC) method has been developed and subsequently validated for the determination of Lacosamide in Bulk and its pharmaceutical formulation. Separation was achieved with a Xterra RP-8 ((Make: Waters Corporation; 150 mmx4.6 mm I.D; particle size 5 µm)) Column and Sodium di-hydrogen phosphate monohydrate buffer (pH adjusted to 3.0 with diluted orthophosphoric acid): Acetonitrile (800:200) v/v as eluent at a flow rate of 1.0 ml/min. UV detection was performed at 230nm. The method is simple, rapid, and selective. The described method of Lacosamide is linear over a range of 12.0µg/ml to 37.85 µg/ml. The method precision for the determination of assay was below 1.0%RSD. The percentage recoveries of active pharmaceutical ingredient (API) from dosage forms ranged from 99.3 to 100.9%. The results showed that the proposed method is suitable for the precise, accurate and rapid determination of Lacosamide in bulk, its dosage forms. Key Words: Lacosamide, RP-LC, Validation, Dosage form.