Payal Chauhan

A simple, accurate, precise and sensitive RP- HPLC method has been developed for the determination of Gatifloxacin and Flurbiprofen Sodium in their pharmaceutical formulation. Chromatographic separation  was carried out on InertsilODS – 3 column (250 mm ×4.6 mm, 5µm ) as stationary phase by using mobile phase consisting of 0.02 M Phosphate buffer (pH 3.5 adjusted with orthophosphoric acid ) : Methanol (80 : 20 v/v). The flow rate was 1.5 ml/min with UV-detection at 245 nm. The retention time was found to be 2.59 min for Gatifloxacin and 5.41min for Flurbiprofen Sodium. The method was validated for various parameters according to ICH guideline. The linear regression analysis data for the calibration plots showed good linear relationship in the concentration range of 30 – 90 µg/ml and 3 – 9 µg/ml and correlation coefficient was found to be 0.9988 and 0.9992 for Gatifloxacin and Flurbiprofen Sodium respectively. The Limit of Detection for Gatifloxacin and Flurbiprofen Sodium were 1.45 and 0.028 respectively. The Limit of Quantification for Gatifloxacin and Flurbiprofen Sodium were 4.39 and 0.28 respectively.

A new simple, rapid, precise, accurate and specific stability indicating method has been developed for the simultaneous estimation of Niacin (NIA) and Lovastatin (LOVA) in tablet dosage form. A chromatographic column used for separation was (250*4.6mm i.d., 5 mm) C18 (Hyperchrome ODS-BP).The mobile phase was 0.02M Disodium hydrogen Phosphate buffer:Acetonitrile (75:25, pH-5) and UV detection of effluent at 237nm.The flow rate was 1ml/min. The retention times of Niacin and Lovastatin were 3.29 min and 4.75 min, respectively. The range of Linearity for Niacin and Lovastatin were 125-325μg/ml and 5-25 μg/ml respectively. The recoveries of Niacin and Lovastatin were found to be in the range of 99.91-100.42 % and 100.03-100.41% respectively. The optimized RP-HPLC method proved to be specific, accurate and robust for the estimation of Niacin and Lovastatin in tablet dosage form. Stability testing study includes the acid hydrolysis, base hydrolysis, oxidation, thermal degradation, and photolysis.