Osman Ahmed

A sensitive and selective RP-HPLC method is described for the determination of stability in Acetaminophen and Tramadol dosage forms. Chromatographic separation was achieved on a C18 column using mobile phase consisting of a mixture of mixed Phosphate buffer pH: 3.4 Acetonitrile (30:70v/v/v), with detection of 236nm and flow rate at 1mL/min. Linearity was observed in the range 100-300 µg /ml for Acetaminophen (r2 =0.994) & 10-30µg /ml for Tramadol (r2 =0.994) for the amount of drugs estimated by the proposed methods was in good agreement with the label claim. No chromatographic interference from tablet excipients was found. The proposed methods were validated. The force degradation of the drugs was assessed by different environmental conditions. Recovery experiments indicated the absence of interference from commonly encountered pharmaceutical additives. The method was found to be precise as indicated by the repeatability analysis, showing %RSD less than 2. All statistical data proves validity of the methods and can be used for routine analysis of pharmaceutical dosage form.

To synthesize and characterize novel thiazolidinone derivatives and screen them for anti tubercular activity. A series of ten 2-(substituted phenyl)-3-[{4-(1-naphthyl)-1, 3-thiazol-2-yl} amino]-5-methyl-1, 3-thiazolidin-4-ones (TM1-TM10) were synthesized from 1-acetyl naphthalene. The synthesized compound, characterized on the basis of satisfactory analytical and spectral (IR, H1NMR, Mass and elemental) data. Studies were carried out for the synthesized compounds which were also evaluated for anti-tubercular activity by using Lowenstein-Jensen (LJ) acid medium and screening by Cup plate method. Rifampicin (Lupin) is used as standard antitubercular agents. The synthesized compounds showed good anti tubercular activity, compared to standard drugs. Two of the compounds TM1 (Ar = 4-nitrophenyl) and TM6 (Ar = 3-fluorophenyl) exhibited significant anti-tubercular activity, as compared to standard drug Rifampicin. We report the successful synthesis of novel thiazolidinones, as well as their spectral characterization, and anti tubercular activity which, for some, is superior to currently used anti-tubercular agents.