Nuzhath Fathima

A simple, rapid, sensitive, accurate, precise spectrophotometric method has been developed for the estimation of Tamsulosin in the presence of Dutasteride  in pharmaceutical formulations. During the course of study, it is observed that acidic solution of the drug formed colored ion-association complexes with Bromo Thymol Blue (BTB) which is soluble in methanol. This property of the drug was followed for the development of spectrophotometric method for analysis of drug. The complex of Tamsulosin in the presence of Dutasteride with Bromo Thymol Blue showed ?max at 430 nm. The linearity range for Tamsulosin in the presence of Dutasteride was 10 µg/ml to 250 µg/ml. Recovery studies gave satisfactory results indicating that none of common additives and excipients interfere the assay method. The molar absorptivity and the sandell sensitivity of the method are found to be 6.6123×104 lit/mole/cm and 0.0067 µg/ml/cm2 respectively. 

A simple, versatile and a new spectrophotometric method is proposed for the estimation of microgram quantities of the drug Megagliptin.  The drug forms a charge transfer (CT) complex with 2,3-Dichlro-5,6-Dicyano-1,4- Benzoquinone (DDQ), the stoichiometry of which is established as 1:1 by Job’s continuous variation method.   The wavelength of maximum absorbance of the CT complex is found to be 385 nm.  The absorbance values of the CT complex increased linearly with the increase in the amount of the drug Megagliptin upto 300 μg/ml.  This suggests that suitability of the method for the determination of the drug in the range 10 μg/ml to 300 μg/ml.  This also indicates the verification of the Beer-Lambert’s Law in this range.  The method is successfully applied to evaluate the assay of commercial tablets in pharmaceutical formulations for Megagliptin and the results agreed very well.  The molar absorptivity and Sandell Sensitivity of the method are 2.346 x 104  lit. mol-1. cm-1  and 0.01818 μg.ml-1.cm2 respectively.