Nadia

In order to gather all the information concerning the nature and the therapeutic uses of the medicinal plants, an ethnobotanical study of the medicinal plants was realized Oran city’s circle, between December 2017 and April 2018. This research was conducted in collaboration with different plant users such as ordinary users (500 people) who were randomly selected (340 women, 160 men). The survey targeted 500 people from the local population, including 423 people preferring medicinal plants with a rate of 84% and 77 people who received treatment in modern medicine with a rate of 16%. The results obtained from the population made it possible to identify 58 medicinal plants which are divided into 34 families, of which three are the most dominant, in particular Lamiaceae (29.55%), Apiaceae (17.96%) and Verbenaceae (14.65%). all listed plants with properties against allergy. the leaves are the most used organs (34%) and the majority of the remedies is prepared as an infusion (58%), the respiratory diseases occupy the first place with a rate of 42%. The results obtained constitute a very valuable source of information for the region studied and for the national medicinal flora. They could be a database for further research in the fields of photochemistry and pharmacology and for the purpose of searching for new natural substances.

Enrofloxacin (EFX) is a third generation Fluoroquinolone with a broad spectrum antibacterial activity. Enrofloxacin hydrochloride is 1-cyclopropyl-7-(4-ethylpiperazin-1-yl)-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxylic acid. A Sensitive, simple and rapid reverse phase high performance liquid chromatographic method was developed for the determination of Enrofloxacin (EFX) in tablet dosage form. The chromatographic separation was performed on a Kromasil C-18 column (250mm x 4.6 mm x 5µ) in isocratic mode using phosphate buffer pH 3:Methanol (40:60 v/v), pH adjusted to 3.0 using orthophosphoric acid as mobile phase at a flow rate of 1.0 ml/min with column temperature 30 OC. The quantification was performed at 280 nm. The method showed good linearity over the concentration range of 5-25 µg/ml with correlation coefficient r2 0.9996. LOD and LOQ was found to be 1.0 and 3.0. The developed RP HPLC method was applied to EFX in tablet dosage form and results were found to be in agreement with the label claim.

Citrullus colocynthis Schrad., traditional Tunisian medicinal plant, showed beneficial effects against oxidative stress mediated diseases, namely its fruits and seeds which contain several compounds with biological activity. The present study reports the antioxidant and the antiproliferative properties of different seed and fruit organic extracts. Antioxidant activity was assessed by the ability to quench the free DPPH and the superoxide anion radicals and inhibit the ABTS cation. Methanol extracts presented the highest DPPH scavenging (seeds IC50 = 0.178 mg/ml; fruit IC50 = 0.223 mg/ml) and superoxide scavenging (seeds IC50 = 28.102 µg/ml; fruits IC50 = 30.793 µg/ml) activities. All extracts inhibited the ABTS radical formation. The most interesting TEAC values were registered with methanol extracts (seeds = 1.225 and fruits = 1.120). Petroleum ether seed extract showed the lower antioxidant action. Seed and fruit organic extracts were also tested for their antiproliferative activity on HT-29 human cell line. All extracts induced a concentration dependent growth inhibition. Petroleum ether seed extract exhibited the higher growth inhibition activity (IC50 = 321 µg/ml), while methanol fruit extract showed the less antiproliferative efficiency (IC50 >500 µg/ml). Data obtained indicate that seeds and fruits constitute an excellent source of effective natural antioxidants and chemopreventive agents.

Generic forms of chemically- synthesized drugs must exhibit chemical identity and be bioequivalent in healthy human subjects. Biologic products are 100- to 1,000-fold larger than chemically synthesized drugs, with sophisticated three-dimensional structures, and can be mixtures of isoforms rather than pure homogeneous entities. Therefore, the development process for biosimilars is more complex than for a true generic and the demonstration of approvability for biosimilars differs from the standard generics approach as it is based on a comparability exercise rather than on demonstration of bioequivalence. This study examines the case of a Low-molecular-weight heparins (LMWHs): enoxaparin which is among smallest biological molecules. Different chemical tests such as Nuclear Magnetic Resonance (NMR), Size exclusion Chromatography, Specific absorbance, stability tests and biological assay (anti-factor Xa activity and anti-factor II a activity) were used for a comparability exercise focusing on quality and structural aspects of enoxaparin biosimilar product compared to the reference product. All tests and comparative studies showed no significant difference. in fact the data observed suggests comparable results even under accelerated conditions of stability study. This study suggests that there is no significant difference in the profile structure and overall studied quality aspects of the reference product compared to the similar biological medicinal product. however specific analytical methods as well as additional biological and pharmacological tests may be used to address their interchangeability.

A simple, specific, selective and accurate stability-indicating reversed phase high performance liquid chromatographic method was developed for simultaneous determination of Diclofenac potassium (DIC), Paracetamol (PCM) and Methocarbamol (MET). An isocratic RP-HPLC was achieved on younglin HPLC system using Varian C18 (250 Χ 4.6 mm i.d, 5 μm particle size) column with the mobile phase containing mixture of Methanol:water (80:20,v/v). The flow rate was 0.8 ml/min and the eluent was monitored at 225nm. The retention times of DIC, PCM and MET were found to be 3.51, 6.42 and 9.90 min respectively. The linearity was established for DIC, PCM and MET in the range of 10-60 µg/ml, 65-390µg/ml, 100-600µg/ml respectively. The percentage recoveries of DIC, PCM and MET were found to be in the range of 99.73%±0.109, 99.59%±0.085 and 99.50%±0.16 respectively. The LOD for DIC, PCM and MET were found to be 0.15, 2.40 and 1.82μg/ml respectively, while LOQ were 0.48, 7.29 and 5.53μg/ml respectively. All three drugs were subjected to acid, alkali, oxidation, and dry heat degradation. The degradation studies indicated DIC, PCM and MET showed degradation in acid, alkaline, H2O2, and in dry heat condition. The degradation products of DIC, PCM and MET were resolved well from the pure drug with significant differences in their retention time values. This method was also successfully employed for simultaneous quantitative analysis of DIC, PCM and MET in bulk drugs and formulations. The developed method is stability indicating and separate degradants and can be used to determine the stability of samples.