Maulik Acharya

Ultra performance liquid chromatography (UPLC) system involves significant technological advances in particle size performance, system optimization, data processing, detector design and control. When all brought together, the specific achievements in each area have created a step-function progress in chromatographic performance. This new technique of analytical separation science uses the principles and practicality of HPLC with increasing the attributes of speed, sensitivity and resolution. Now a day’s pharmaceutical industries are in search of new ways to reduce cost and time for analysis of drugs. Analytical laboratories are not exception in this trend. Ultra high performance liquid chromatography (UPLC) with better resolution, assay sensitivity and high sample throughput allows a greater number of analysis to be performed in a shorter period of time and it also impart cost effective advantage over HPLC analysis. So that conventional assay was transferred and optimized for UPLC system. This review introduces the theory of UPLC, and involves some of the most advanced work in the field.

  In ancient Greek ‘Nano’ means dwar. Nanotechnology is the creation and utilization of materials, devices, and systems through the control of matter on the nanometer-length scale, i.e. at the level of atoms, molecules, and supramolecular structures. These technologies have been applied to improve drug delivery and to overcome some of the problems of drug delivery for cancer treatment. The magic of nanoparticles mesmerize everyone because of their multifunctional character and they have given us hope for the recovery from this disease. Nanoparticles can be used to deliver hydrophilic drugs, hydrophobic drugs, proteins, vaccines, biological macromolecules, etc. They can be formulated by aptamer conjugation for targeted delivery to the lymphatic system, brain, arterial walls, lungs, liver, spleen, or made for long-term systemic circulation. Therefore, numerous protocols exist for synthesizing nanoparticles based on the type of drug used and the desired delivery route. In modern medicine technologies the oral administration of solid forms is the preferred route for drug delivery. Thus, in pharmaceutical applications, size, shape and morphology of the solid particles are important because they can affect the solubility as well as bioavailability of the drug particles.