M.Gopala Krishna

A new RP-HPLC method for the assay of mebendazole in in unadulterated and in tablet measurements has been developed and validated. The present chromatographic study was carried on μ Bondapak® C18 (250X4.6 mm), 5μm with a flow rate of 1.0mL/min of mobile phase and UV detection at of 218nm and ambient column temperature with mobile phase of NaH2PO4 buffer(pH-5.0) and acetonitrile in the ratio of 65:35%v/v as the mobile phase. The retention time for mebendazole were found to be 2.60mins respectively. The developed RP-HPLC method was validated as per ICH guidelines and was found to be suitable for pharmacokinetic studies.