Lincy Davin

A total of 25 different types of actinomycetes were isolated from the soils of marine sediment soil. All the isolated actinomycetes were characterized and identified based on the morphological, biochemical, cultural characteristics. Both primary and secondary screening methods were used to screen actinomycetes for antibacterial activity. The result of the screening revealed that all the isolates were against bacterial culture. But the best strain was found to be Streptomyces sp as they showed broad spectrum activity with big zone of inhibition, even though the strain Staphylococcus and Streptomyces sp showed augmented antibacterial activity against all the tested human bacterial pathogens. Comparatively, when they were treated with pathogenic microorganisms all the isolates produced to maximum and minimum zone of inhibition with its responsible broad spectrum of bioactivity. Moreover, LAM-4 and LAM-11 strains were clearly showed significant activity against both Staphylococcus aureus and S. agaricus. From the HPLC peaks which had antimicrobial activity of Streptomyces sp was identified to be at 3.296. This is similar to oxohexaene antibiotic whereas, the antimicrobial compound of Streptomyces sp3 showed a retention time of 3.233 on HPLC, this peak was similar to Cephalexin. From the results highlight the most of the isolates inhibited growth of the Gram negative bacteria tested. All the antibiotic producing actinomycetes were isolated at different temperatures from marine soil. These microorganisms might have potential to produce a number of the most important medicines constantly developed. Key words- Actinomycetes, pathogenic organisms, HPLC