Kuppusamy Periyasamy

The objective of this study was to determine the efficacy of Plumbagin on attenuating malondialdehyde (MDA) and nitric oxide (NO), along with augmenting the endogenous antioxidant status in DMBA induced mammary carcinoma. Breast cancer was induced by providing 1ml of 7, 12 dimethyl benze (a) anthrecene(DMBA) 20mg/kg of body weight through orally and tissue antioxidants status along with histopathological studies were determined as per standard procedures. Western blot was also analyzed to determine the protein expression in all the experimental groups. Altered antioxidant status and upsurge free radical generation especially nitric oxide was observed in DMBA induce group when compared to control. This increase NO level was well supported by increase protein expression of iNOS and eNOS. However the altered antioxidant status was corroborated with decrease GSH protein expression. To support the detrimental effect of DMBA histopathology study showed neovasularization, presence of uniformly malignant ductal epithelial cells growing in vague cribiform pattern, necrosis formation along the tumor, and cell destructions. However, Plumbagin when administered at 4 mg/kg body weight showed attenuated free radical generation and upregulated the antioxidant activity. The increase free radical generation especially NO is also plays a pivotal causative role in breast cancer. Therapeutic efficacy targeting the antioxidant system could play a pivotal role in the treatment of breast cancer.

Breast cancer is one of the most serious problems in oncology. It is a leading cause of death among women in many countries. Environmental factors, of either biological or chemical origin, may act as initiators and promoters of  the carcinogenesis. Chemical carcinogens such as 7,12-dimethylbenz[a] anthracene [DMBA],benz[a]pyrene [BP], and N-nitroso-N-methylurea are commonly employed to initiate and promote neoplastic transformation in experimental animals. DMBA is well established as a highly potent carcinogen. Cancer chemoprevention is recognized as the most promising and novel approach to prevent, inhibit or reverse the carcinogenic processes by intervention with natural products or synthetic chemical substances. Taurine is a sulfur containing beta amino acid with a wide range of vital, biological functions, ranging modulation, cell membrane stabilization to bearing an antioxidant and scavenging agent. In from neuro the last decade it has been widely used in the field of oncology as a chemo protective agent against hepatocarcinogenesis and colon carcinogenesis. The aim of this study was to get insight into the process of chemo resistance acquisition for a better understanding of the breast cancer therapy. Therefore, the current study has been undertaken to examine the effect of taurine on DMBA induced breast cancer in rats. At the end of the experimental period, the homogenized breast tissue was investigated and recorded the body weight, tumor weight and antitumor activity of Taurine in the experimental rats. Overall, these results suggested that the taurine treatment provided antioxidant defense with strong chemopreventive activity against the genesis of DMBA-induced mammary carcinoma.