Jose Gnana Babu C

A simple, specific, accurate, precise and sensitive RP- HPLC method has been developed for the rapid estimation of Alogliptin in bulk and its formulations. The chromatographic separation was carried on Phenomenex Gemini-NX-5 µm C18(2) 110A, LC Column 250 x 4.6 mm, using Acetonitrile:1-octasulphonoic acid (0.005mM) at pH-5 [60:40] (v/v) as mobile phase, at a flow rate of 1.0 ml/min.  The detection was carried out at 220 nm and drug eluted with a retention time of 3.48 min. Beer’s law was obeyed in the concentration range of 2-10?g/ml with correlation coefficient 0.9995. The method has been validated according to ICH guidelines for specificity, linearity, accuracy, precision, robustness, ruggedness, LOD and LOQ. The method was found to be specific, accurate, and precise, robust, rugged and sensitive. The developed method was good linearity, novel, rapid for the estimation of Alogliptin in bulk and tablets dosage form. Thus it can be employed for the routine analysis.

A simple, specific, quick, isocratic Reversed Phase High Performance Liquid Chromatographic method was developed and validated for the analysis of Noscapine. RP-HPLC method was developed on a Symmetry C-8 (4.6 × 150 mm), 3.5 µm particle, reversed-phase column. The mobile phase was 0.1% octane sulphonic acid (pH- 3): acetonitrile, 40:60 (v/v) at a flow rate of 0.8 ml/min. and the eluate was monitored at 260 nm. The retention time of the drug was found to be 2.314 min. The method was linear over the range of 4-8 μg/ml with a regression coefficient of 0.999 and validated with respect to accuracy, precision, linearity, and specificity, limit of detection and limit of quantization as per the guidelines of International Conference for Harmonization (ICH). This method can be used in the industries for determination of Noscapine to analyze the quality of formulation without interference of the excipients.

A new, simple and sensitive UV-spectrophotometric method was developed for the determination of Benfotiamine in bulk and tablet dosage form. This method, involves the measurement of absorbances of Benfotiamine at the wavelength of 244nm. 0.1M HCl was used as solvent. Linearity was observed in the concentration range of 3-18µg/ml with correlation coefficient 0.999. The accuracy of the method was confirmed by recovery studies of tablet dosage forms and was found to be 99.32%-100.42% for Benfotiamine. The method showed good reproducibility and recovery with %RSD lessthan 2.0. The LOD and LOQ of Benfotiamine was found to be 0.051μg/ml and 0.155μg/ml. Results of the analysis were validated for accuracy, precision, LOD, LOQ and were found to be satisfactory. Thus the developed method was found to be simple, sensitive, rapid, precise, accurate and cost effective quality control tool for the routine analysis of Benfotiamine in bulk and tablet dosage form.