Indrajeet Singhvi

Enrofloxacin (EFX) is a third generation Fluoroquinolone with a broad spectrum antibacterial activity. Enrofloxacin hydrochloride is 1-cyclopropyl-7-(4-ethylpiperazin-1-yl)-6-fluoro-4-oxo-1, 4-dihydroquinoline-3-carboxylic acid. A Sensitive, simple and rapid reverse phase high performance liquid chromatographic method was developed for the determination of Enrofloxacin (EFX) in tablet dosage form. The chromatographic separation was performed on a Kromasil C-18 column (250mm x 4.6 mm x 5µ) in isocratic mode using phosphate buffer pH 3:Methanol (40:60 v/v), pH adjusted to 3.0 using orthophosphoric acid as mobile phase at a flow rate of 1.0 ml/min with column temperature 30 OC. The quantification was performed at 280 nm. The method showed good linearity over the concentration range of 5-25 µg/ml with correlation coefficient r2 0.9996. LOD and LOQ was found to be 1.0 and 3.0. The developed RP HPLC method was applied to EFX in tablet dosage form and results were found to be in agreement with the label claim.

To synthesize and characterize novel thiazolidinone derivatives and screen them for anti tubercular activity. A series of ten 2-(substituted phenyl)-3-[{4-(1-naphthyl)-1, 3-thiazol-2-yl} amino]-5-methyl-1, 3-thiazolidin-4-ones (TM1-TM10) were synthesized from 1-acetyl naphthalene. The synthesized compound, characterized on the basis of satisfactory analytical and spectral (IR, H1NMR, Mass and elemental) data. Studies were carried out for the synthesized compounds which were also evaluated for anti-tubercular activity by using Lowenstein-Jensen (LJ) acid medium and screening by Cup plate method. Rifampicin (Lupin) is used as standard antitubercular agents. The synthesized compounds showed good anti tubercular activity, compared to standard drugs. Two of the compounds TM1 (Ar = 4-nitrophenyl) and TM6 (Ar = 3-fluorophenyl) exhibited significant anti-tubercular activity, as compared to standard drug Rifampicin. We report the successful synthesis of novel thiazolidinones, as well as their spectral characterization, and anti tubercular activity which, for some, is superior to currently used anti-tubercular agents.