Hemant kumar

To develop a fast and sensitive  UV spectrophotometric method for the quantitative estimation of Formaldehyde in Rivastigmine tartrate drug substances and validate as per ICH guidelines. The method was based upon the observation, that a characteristic colourresults upon addition of  solution of Pentane-2,4-dione , also known as acetylacetone . In acetic acid and ammonium acetate buffer condition, acetylacetone and formaldehyde react to form dimethyl pyridine. Dimethyl pyridine is slightly yellow and its absorption maximum in aqueous solution is λ 420 nmin Rivastigmine tartrate drug substance.The developed method resulted in Formaldehyde exhibiting linearity in the range 0.975 to 234 µg/g. The Intraday and interday precision is exemplified by relative standard deviation of 0.562 % and 0.757%. Percentage Mean recovery was found to be in the range of 98‐101%, during accuracy studies. The limit of detection (LOD) and limit of quantitiation (LOQ) were found to be 1.3 µg/g and 3.9 µg/g respectively.The present work was aimed to develop a visible spectrophotometric method, which is simple, sensitive, accurate and cost effective to evaluate the quality of the bulk drugs.

Peptic ulcer is the areas of degeneration and necrosis of gastrointestinal mucosa exposed to acid peptic secretion. Some other factors, such as inadequate dietary habits, excessive ingestion of non-steroidal anti-inflammatory agents, stress, hereditary predisposition and infection by Helicobacter pylori, may be responsible for the development of peptic ulcer. The several herbal formulations derived from Ayurveda and its additional systems of medicine, not yet to be scientifically validated that they have exhibited anti-ulcer activity. Our present study was to evaluate the ulcer protective activity of ethanolic extract of Gynocardia odorata (G.   ordata) seeds on cold restraint and ethanol induced ulcer model. Five groups were taken (n=6). First groups serve as negative control (Without any treatment). Second groups were treated with vehicles only. Third groups were treated with some standard marketed drugs (Ranitidine and sucralfate). Fourth and fifth groups were treated with ethanolic seed extract of G. odorata in two increasing dose (250 and 500mg/kg body weight p.o respectively). Both the group showed significant ulcer protective activity in a dose dependent manner.