Ekambaram Jayachandran

A simple, fast and precise reverse phase, isocratic HPLC method was developed for the separation and quantification of perindopril and indapamide in pharmaceutical dosage form. The quantification was carried out using YMC Column (150 x 4.6mm, 3µ particle size) and mobile phase comprised of ammonium dihydrogen phosphate pH 2.5 and acetonitrile in the ratio of 60:40% v/v and degassed under ultrasonication. The flow rate was 1.0 mL/min and the effluent was monitored at 230 nm. The retention time of perindopril and indapamide were 2.4 and 4.2 min respectively. The method was validated in terms of linearity, precision, accuracy and specificity. Linearity of perindopril and indapamide were in the range of 48 to 112 μg/mL and 15 to 35 μg/mL respectively. The proposed method is suitable for simultaneous determination of perindopril and indapamide in pharmaceutical dosage form. Key words: Perindopril and Indapamide, RP-HPLC, Validation.

Two simple and sensitive ion-pairing spectrophotometric methods have been developed for the assay of saxagliptin in pure form and in tablet dosage form. The developed methods involve formation of yellow colored chloroform extractable ion-pair complexes of the drug with Bromocresol Green (BCG) and Bromothymol Blue (BTB) in acidic medium. The extracted complexes showed absorbance maxima at 420, 415 nm for BCG, BTB, respectively. Beer’s law is obeyed in the concentration ranges 10-50 μg ml-1 in both methods with molar absorptivity of 3.164 x 103, 4.305 x 103, L mole-1 cm-1 for BCG and BTB, respectively. These methods have been successfully applied for the assay of drug in tablets. Results of analysis were validated statistically and through recovery studies.