B. Rajkamal

In the present study a simple isocratic reverse phase HPLC method was developed for the estimation of rilpivirine in pharmaceutical formulation. The separation was carried out using a column of Zorbax Eclipse XDB-C18, 250x4.6mmi.d with 5micron particle size. The mobile phase comprises of 0.03M di potassium hydrogen orthophosphate with pH adjusted to 2.5 using dilute ortho-phosphoric acid (mobile phase solvent-A) and acetonitrile (mobile phase solvent-B) in the ratio of 15: 85 (v/v).The flow rate was 1.0 ml/min and the effluents were monitored at 284 nm. The retention time was 7.19 min. The detector response was linear in the concentration range of 100-300µg/ml. The respective linear regression equation being Y= 28817.742X-14741.2. The limit of detection (LOD) and limit of quantification (LOQ) for rilpivirine were found to be 0.05µg/ml and 0.15 µg/ml respectively. The assay was found to be 99.85%.The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of rilpivirine in its pharmaceutical dosage form.

The present study was an attempt to prepare and evaluate Zolmitriptan 9 different oral disintegrating tablets using superdisintegrants like SSG, Crospovidone and Croscarmellose sodium. Formulations were evaluated for their micromeretic properties and post compression studies and found to be within the limits. Based on the disintegrating time and dissolution studies F9 was found to be best formulation. It was found that the sodium starch glycolate is much more effective than the other super disintegrating agents in the preparation of Zolmitriptan oral disintegrating tablets. DSC and FTIR data revealed that no interactions takes place between the drug and polymers used in the optimized formulation.