Determination of Sildenafil Citrate In Pharmaceutical Dosage Forms by Reverse Phase High Performance Liquid Chromatography Method

The main aim of the present study was to develop and validate a simple, rapid and sensitive assay method for sildenafil citrate in pharmaceutical dosage forms by reverse phase high performance liquid chromatography method. Determination Of Sildenafil Citrate was determined by reverse phase HPLC using Potassium dihydrogen phosphate Buffer (pH 7.0):ACN(45:55) and column  Inertsil ODS-3V 5μ (150*4.6mm) as a stationary phase and  the chromatogram of Sildenafil Citrate has shown in figure 10 and peak was observed at 228 nm which was selected as a wavelength for quantitative estimation. After development of the method, it was validated for system suitability, specificity, linearity, precision, accuracy, robustness and solution stability studies. The results system suitability was found to be within the limits. The limit were not more than RSD