Method Development and Validation for the Simultaneous Estimation of Propranolol Hydrochloride and Etizolam by Reverse Phase High Performance Liquid Chromatography

RP-HPLC method has been developed for the quantitative estimation of Propranolol hydrochloride and Etizolam using C 18 column and a mobile phase consisting of Methanol and Water in the ratio 70:30. The mobile phase was pumped at a rate of 1ml/min and detection was carried out at 281.5 nm(iso-absorptive point). The linearity was found to be in range of 5-50µg/ml and 0.5-5µg/ml with regression coefficient (r2=0.999, and r2=0.997) for Propranolol hydrochloride and Etizolam respectively. The peaks obtained were sharp having baseline separation with a retention time of 2.35and 6.57min for Propranolol hydrochloride and Etizolam respectively.The LOD was found to be at the concentration of0.1µg/ml and 0.025µg/ml for Propranolol hydrochloride and Etizolam respectively. The percentage recovery was found to be 98.5% to 100.58% for Propranololhydrochloride and 95% to 106.6% for Etizolam. The method was validated statistically.