Observation of Cytotoxic Activity and Antimicrobial Effect of Crude Extracts of Diospyros peregrina gurke

This dissertation describes the biological investigations of Diospyros peregrina gurke; a plant belonging to the family Ebenaceae.Present study was designed to test the cytotoxic activity and antimicrobial effect of crude extract. The study protocol consists cold extraction of powdered unripe fruit and seed of Diospyros peregrina gurke for cytotoxicity effect using Brine shrimp bioassay lethality and tested against four Gram negative and three Gram positive bacteria for antimicrobial activity. From the results of the brine shrimp lethality bioassay it can be well predicated that crude extracts of Diospyros peregrina gurke possess cytotoxic principles (with LD50  9.12011µg/ml and 10.8893µg/ml).For antimicrobial activity, the extracts were tested against standard microbial strains of gram positive and gram-negative, by means of Agar-Disc Diffusion Method and minimum inhibitory concentration (MIC) was noted.Although, both extracts were found to be effective in inhibiting pathogens to varying degrees to the tested organisms, the unripe fruit pulp extract of Diospyros peregrina gurke was found to be more effective than seed extract of Diospyros peregrina gurke .When both extracts were used in combination, they have shown strong synergistic effect against all the pathogens tested in the present study. Plant extracts  can be used to source antitumor and antibiotic substances for possible treatment.